雄激素诱导大鼠阴茎细胞增殖的研究

Androgen induction of cell proliferation in rat penis

本研究试图探讨雄激素补充治疗能否诱导去势大鼠阴茎勃起组织细胞增殖。 30只成年SD雄性大白鼠随机分为对照组、去势组及补充组。补充组术后 2周肌注十一酸睾酮 (13.7mg/kg) ,并分别于补充治疗后 2 4、48、72及 96h处死 ,最后处死对照组及去势组动物 ,取中段阴茎海绵体 ,用免疫组化技术 (SP法 )检测其细胞增殖情况。发现正常情况下阴茎组织中存在中等强度的细胞增殖 (39.8± 3.47) ,去势 2周后几乎无细胞新生 (P <0 .0 1) ,睾酮补充 48h后即诱导DNA合成 (2 1± 2 .2 9) ,96h后增殖细胞显著增多 (95 .2± 9.6 5 ,P <0 .0 1)。增殖细胞包括平滑肌细胞、成纤维细胞及内皮细胞等。

This study was aimed to investigate whether androgen replenishment could induce cellular proliferation in erectile tissue of castrated rats.In our study,thirty adult male SD rats were randomly divided into 3 groups:controls,castrates and castrates given Testosterone Undecanoate injection (13.7 mg/kg)2 weeks after surgery.In the third group,rats were sacrificed at 24,48,72 and 96 hours after androgen replacement.Then,rats in other groups were all killed.The middle segments of penis were harvested and cell proliferation in them was detected by using strepavidin peroxidase immunohistochemistry techniques(SP method).The results showed that there was a modest baseline level of proliferation(39.8±3.74) in the penis of uncastrated rats,while castrated rats seldom showed new nuclei( P <0.01).DNA synthesis occurred as early as 48 hours after testosterone replenishment.And the cell proliferation significantly enhanced 96 hours after replacement(95.2±9.65, P <0.01).Various types of cells were found to respond to testosterone,including myocytes,fibrocytes ad endothelial cells,etc.Our finding demonstrated that testosterone could induce multicellular proliferation in erectile tissue of castrated rats.