核磁共振在生物代谢研究中的应用

Applications of NMR in biological metabolic research

核磁共振已成为生物代谢研究的有力手段。它是完全非侵入、无损伤的探测方法。因此能够在生理条件下,在分子水平上研究生物体的代谢过程,所以它优于其他传统方法。 利用NMR的重要参数——化学位移、纵向弛豫时间及横向弛豫时间,可以对代谢过程和反应物及产物的结构、浓度、输运和分布等进行观测和跟踪。~1H、^(31)P和^(13)C等核的NMR谱已广泛用于代谢研究。

The nuclear magnetic resonance has become a powerful means of studying biological metabolism in non-invasive and non-destructive way. Being used to study the metabolic processes of living system in normal physiological conditions as well as in molecular level, the method is better than other conventional approaches.Using important parameters such as NMR-chemical shifts, longitudinal relaxation time and transverse relaxatioa time, it is possible to probe the metabolic processes as well as conformation, concentration, transportation and distribution of reacting and resulting substances. The NMR spectroscopy of 1H, 31P and 13C nuclei has already been widely used in metabolic researches.