内蒙古呼伦贝尔泡状蚴(Alveolaris hulunbeierensis)结构的观察

Histologcal observation on Alveolaris hulunbeierensis in the liver of Microtus brandti in northeast Inner Mongolia,China

本文报告在我国内蒙古东北部呼伦贝尔草原布氏田鼠 (MicrotusbrandtiRadde)肝脏发现的呼伦贝尔泡状蚴(AlveolarishulunbeierensisTangetal ,2 0 0 1)的详细结构。它的母囊充满上下两部分结构不同的胚组织。各有数处胚细胞发生中心 ,其中有许多具多细胞核的胚母细胞的增生 ,由它们分裂许多能移动的胚细胞向母囊外迁移。各胚细胞团到宿主肝组织中 ,立刻被宿主的结缔组织细胞所包围 ,形成小雏囊。已成长的泡囊内没有多细胞核的胚母细胞 ,但有许多胚细胞团 ,它们也是大量分散地向囊外移行如同母囊一样。本寄生虫具有泡状蚴的基本特征 ,如泡囊内有行无性增殖的胚细胞组织 ,增生的胚细胞向囊外转移及引起宿主免疫反应 ,受白细胞、淋巴细胞及结缔组织的包围产生新泡囊 ,等等。但本虫种泡状蚴无论在早期母囊结构 ,或雏囊形成方式 ,均与具泡状蚴结构的多房棘球绦虫 (EchinococcusmultilocularisLeuckart,186 3和西伯利亚棘球绦虫 (EchinococcussibiricensisRauschandSchiller,195 4)的幼虫期十分不同。由于呼伦贝尔泡状蚴的成虫期尚未获得 ,故且先以呼伦贝尔泡状蚴命名 (唐崇惕等 ,2 0 0 1)。

The structure of Alveolaris hulunbeierensis Tang et al 2001 is different from the alveolar cysts of Echinococcus multilocularis Leuckart,1863 and Echinococcus sibiricensis Rausch and Schiller,1954 All of them were collected from the livers of field voles,Microtus brandti Radde in Hulunbeier Pasture,northeast Inner Mongolia,China in 1998～2000 This paper reports the results of histological observation on the Alveolaris hulunbeierensis Livers of ten positive voles were fixed in 10% formalin solution 5 of them were made into serial paraffin sections respectively,and were stained with PAS reaction method The mother cyst of Alveolaris hulunbeierensis had unique structure of thick wall and solid alveolar cyst containing two parts (upper and lower) of germinal tissue Each part of them was composed of a central cavity and large area of germinal tissue The proliferations of many large mother germinal cells containing several nuclei were seen in these cavities and germinal tissues Mother germinal cells divided into many germinal cells,and lot of germinal cells masses metastasized off from mother cysts into host reaction tissue and normal liver tissue,in where they were surrounded by much leukocytes and collagenous fibroblasts,and many small early brood capsules were formed The undifferentiated embryonal tissues occured in the lumen of late mother cyst There were no germinal cells layers on the inner surface of the cyst walls of mother cyst and mature brood capsules Many germinal cells masses also metastasized off from the cavities of mature alveolar cysts into host tissue and also were surrounded by host leukocytes and collagenous fibre to form the new brood capsules