摘要
目的 分析不同幽门螺杆菌 (H .pylori)培养滤液对胃上皮细胞端粒酶活性的影响。 方法 分别以来自胃癌患者和来自慢性浅表性胃炎患者的cagA+ vacAs1a/m2和来自慢性浅表性胃炎患者的cagA+ vacAs1b/m2、cagA-vacAs1a/m2的H .pylori培养滤液与胃上皮细胞共孵育 ,然后撤除刺激物继续培养 ,观察不同时间的DNA合成速率和端粒酶活性。 结果 cagA+ vacAs1a/m2和cagA+ vacAs1b/m2Hp培养滤液与胃上皮细胞共孵育 6h ,诱导了胃上皮细胞端粒酶活性表达上调 ,DNA合成速率增加 ,cagA+ vacAs1a/m2的作用明显强于cagA+ vacAs1b/m2 ,撤除刺激物继续培养 2 4h后 ,其改变得以逆转 ;cagA-vacAs1a/m2H .pylori培养滤液与胃上皮细胞共孵育 ,其端粒酶活性和DNA合成速率无明显改变。 结论 不同H .pylori培养滤液对胃上皮细胞端粒酶活性的影响各异 ,cagA+
Aim To analyze the effect of different Helicobacter pylori(H.pylori) culture supernatant on telomerase activity of gastric epithelial cells in vitro.Methods Culture supernatant of cagA + vacA s1a/m2 H.pylori from gastric cancer (GC) and chronic superficial gastritis (CSG) patients,as well as culture supernatant of cagA + vacA s1b/m2 and cagA - vacA s1a/m2 H.pylori from CSG patients was used as stimulator and was incubated with gastric epithelial cells,respectively.The stimulator was removed after 6 hours.DNA synthesis rate of gastric epithelial cells was examined by 3H TdR,and telomerase activity was measured by TRAP ELISA at different time.Results Telomerase activity and DNA synthesis rate of gastric epithelial cells increased by induction of culture supernatant from cagA + vacA s1a/m2 and cagA + vacA s1b/m2 H.pylori. Moreover,the action of cagA + vacA s1a/m2 was stronger than that of cagA + vacA s1b/m2,but after removing stimulator for 24 hours,the changes were reverted.Culture supernatant from cagA - vacA s1a/m2 H.pylori did not significantly alters telomerase activity and DNA synthesis rate of gastric epithelial cells.Conclusions Culture supernatant of different H.pylori has different effect on telomerase activity of gastric epithelial cells,cagA + vacA s1a/m2 H.pylori cytotoxins may up-regulate telomerase activity expression in gastric epithelial cells.
出处
《中国人兽共患病杂志》
CSCD
北大核心
2002年第1期22-25,共4页
Chinese Journal of Zoonoses
基金
广东省科委自然科学基金资助项目 (No.970 46 )
