卡氏肺孢子虫肺炎大鼠模型的比较研究

Comparative study on the rats models of Pneumocystis carinii pneumonia

目的 比较用SpragueDawley(SD)大鼠和Wistar大鼠建立卡氏肺孢子虫肺炎 (PCP)模型的差异。 方法 选用SD大鼠和Wistar大鼠 ,随机分为实验组和对照组 ,免疫抑制诱导建立动物模型 ;收集肺组织和支气管肺泡灌洗液 (BALF) ,分别制成肺印片和BALF涂片 ,作Giemsa染色 ,镜检卡氏肺孢子虫滋养体和包囊。结果 共收集实验组SD大鼠和Wistar大鼠的肺组织及BALF标本各 2 8份 ,经Giemsa染色后 ,在肺印片中查见Pneumocystiscarinii(Pc)虫体的阳性率分别为 89.2 9%和10 0 % ,两者之间无显著性差异 (P >0 .0 5 ) ;BALF阳性率分别为 6 0 .71%和 78.5 7% ,两者之间亦无显著性差异 (P >0 .0 5 )。而同种大鼠的肺印片与BALF涂片 ,其阳性率之间有显著性差异 (P <0 .0 5 ) ,且肺印片的检出率均显著高于BALF涂片。同种大鼠不同肺叶的肺印片 ,其阳性率之间无显著性差异 (P >0 .0 5 )。

Aim To compare Sprague Dawley(SD) rats with Wistar rats in rat model of Pneumocystis carinii pneumonia.Methods SD and Wistar rats were divided into experimental group and control group randomly,and were induced by immunosuppression.Lung tissues and bronchoalveolar lavage fluid (BALF) were collected.Smears were made from lung tissues and BALF sediments,were stained by Giemsa,and were examined for identifying the cysts and trophozoits of P.carinii.Results 28 samples were collected in the experimental group in lung tissues and BALF respectively.The positive rates of P.carinii organisms were 89.29% and 100% in lung smears,and were 60.71% and 78.57% in BALF slides,respectively.The difference between the SD and Wistar rats was no significant (P>0.05).The positive rates in lung smears were higher than that of BALF slides and the difference between the two methods was significant (P<0.05).The positive rates in lung smears from different lobes were no significant (P>0.05).Conclusions SD and Wistar rats had no significant difference in animal model of P.carinii.