福建省婴幼儿腹泻轮状病毒G型和P型的分型研究

Serotyping and genotyping of G and P types of rotavirus from infantile diarrhea in Fujian Province

目的 研究福建省婴幼儿轮状病毒G型和P型的分型。方法收集福建省 5个地区婴幼儿腹泻粪便标本 32 7份 ,应用聚丙烯酰胺凝胶电泳、单克隆抗体及逆转录—聚合酶链反应进行轮状病毒检测及G型和P型分型研究。结果 检出 184份阳性标本 ,阳性率 5 3.2 %。在 15 3份阳性标本中 ,G1型 118株 (77.1% ) ,G2型 13株 (8.5 % ) ,G3型 7株 (4 .6 % ) ,G4型 10株 (6 .5 % ) ,混和型 3株 ,未定型 2株 ;Ga型未检出 ;福州、南平、泉州、厦门地区均以G1型为主 ,但漳州地区全部是G2型 ;对G型定型的 48份阳性标本进行P型分型 ,其中P[8]型 2 4株 (5 0 0 % ) ,P[4 ]型 13株 (2 7 0 % ) ,P[6 ]5株 (10 4% ) ,P[9]、P[10 ]型未检出 ,未定型 6株 ;G型与P型组合以G1P[8]最常见 ,G2P[4 ]次之。结论 福建省近年来大部分地区婴幼儿轮状病毒流行型别以G1P[8]为主 ,个别地区以G2P[4

Aim To investigate the distribution of G and P types of rotavirus in Fujian Province.Methods 327 stool specimens collected from infants with diarrhea in five districts of Fujian Province were tested for rotavirus and G,P typing by PAGE,MAbsELISA and RT-PCR.Results Rotavirus is detected from 184 out of 327(53.2%) specimens.Among 153 strains of rotavinus performing with G typing,118 strains (77.1%) were G1 type,13 (8.5%) G2 type,7 (4.6%) G3 type,10 (6.5%) G4 type,3 mix types and 2 nontypable,G9 type not found;G1 was the predominant type in the districts of Fuzhou,Nanping,Quanzhou,and Xiamen,but all strains from Zhangzhou were G2.Among 48 strains performed with P typing,24 strains (50 0%) were P,13 (27 0%) P,5 (10 4%) P,6 nontypable,respectively.P and P not found.G1P was the most common combination and G2P the next.Conclusion Recently G1P is the predominant infantile rotavirus in majority of districts in Fujian Province,with exception that G2P is the most common type in Zhangzhou district.