链霉亲和素产生菌培养条件的研究

STUDY OF CULTURE CONDITIONS FOR STREPTOMYCES

目的通过对分泌链霉亲和素 (streptavidin)的菌株L 183最佳培养条件的研究 ,为提高链霉亲和素的产量及其规模生产打下了基础。方法选用 3种种子培养基和 5种发酵培养基 ,通过检测种子菌体湿质量、间接ELISA检测发酵液中链霉亲和素的效价 ,筛选出菌体生长快的种子培养基及链霉亲和素产量高的发酵培养基。结果B号种子培养基、2 #发酵培养基最适合L 183的生长和链霉亲和素合成 ;不同菌种接种量对链霉亲和素的产量也有明显影响 ,在 140ml发酵液中 ,种子接种量为 0 .3ml,链霉亲和素产量可达 111mg/L。 结论本实验筛选的培养基价格低廉 ,且L 183生长快、链霉亲和素产量高 。

ObjectiveTo explore the best culture condition for L 183 which secreted streptavidin and to establish a base for a large scale production of streptavidin. MethodsThree liquid seed culture media and 5 ferment culture media were used and the best media were chosen by examining wet mass of L 183 and production of streptavidin.ResultsSeed culture medium B and ferment culture medium 2# were suitable for the growth and streptavidin synthesizing of L 183. Streptavidin production was also affected by inoculum size of the strain. Streptavidin production can reach 111 mg/L when 0.3 ml packed cells were inoculated in 140 ml culture media 2#. ConclusionThe media chosen by this study was low cost and suitable for the growth and streptavidin yield of L 183 . They were first chosen media for streptavidin production in large scale.