成骨细胞与生物衍生材料联合培养的实验研究

EXPERIMENTAL STUDY OF PERIOSTEAL OSTEOBLASTS COCULTURE WITH FREEZE-DRIED DEMINERALIZED BONE MATRIX

目的 探索冻干脱钙骨基质 (FDBM)作为组织工程骨支架的可行性。方法 取兔颅骨外膜的成骨细胞 ,经传代培养后作为种子细胞与 FDBM于体外联合培养 ,通过对复合物相差显微镜、光镜及扫描电镜等观察 ,了解细胞在材料中的生长情况。结果 经系统处理后的 FDBM呈现不规则的网 -孔结构 ,其孔隙直径为 10 0～40 0 μm,孔隙率为 70 %。体外复合培养 8小时 ,成骨细胞即开始贴附于 FDBM网架上 ;复合培养 7天 ,分布于支架材料上的成骨细胞迅速分化增殖 ,分泌细胞外基质并形成钙结节。结论

Objective To investigate the feasibility of freeze dried demineralized bone matrix (FDBM) as scaffold material in bone tissue engineering. Methods Osteoblasts which were isolated from cranial periosteum of New Zealand rabbits were cultured as the seeding cells, then the cells were cocultured with heterogenous FDBM in vitro. The cell material complex was observed under phase microscope, light microscope and electronic scanning microscope in order to evaluate the interaction between cells and FDBM. Results Eight hours after coculture, the osteoblasts adhered to FDBM scaffolds. Seven days later, the osteoblasts differentiated and proliferated in FDBM network. Extracellular matrix was secreted and calcium nodes were formed among osteoblasts. Conclusion FDBM is a good scaffold material for the bone tissue engineering.