转基因表皮细胞在生物膜上种植的实验研究

EXPERIMENTAL STUDY OF THE TRANSFERRED KEROTINOCYTES TRANSPLANTED ON BIOMEMBRANE

目的 检测转基因表皮细胞在人工真皮和生物硅膜上能否生长 ,是否适合作为表皮细胞移植的载体。方法 分别将转基因表皮细胞种植于人工真皮和生物硅膜上 ,培养 2周 ,通过光镜和扫描电镜 ,观察细胞在两种生物膜上的生长情况。结果 种植于人工真皮上的表皮细胞 2～ 3天开始破裂、死亡。种植于生物硅膜上的表皮细胞 ,1周时扫描电镜下见表皮细胞在生物硅膜表面贴附较好 ,有伪足形成 ,提示细胞生长良好 ;2周时光镜下见生物硅膜上的表皮细胞形态仍正常 ,增殖良好。

Objective To search an ideal carrier of transferred keratinocytes for transplantation. Methods The transferred keratinocytes were seeded on the surfaces of the artificial dermis and the silicone membrane and cultured in vitro for 2 weeks. The growth of the keratinocytes was observed by microscope and scanning electron microscope. Results The keratinocytes implanted on the artificial dermis began to rupture and died after 2 to 3 days. While the keratinocytes adhered well on the surface of silicone membrane with pseudopodia formation after 1 week under scanning electron microscope, and the cells kept normal morphological and proliferative properties 2 weeks later. Conclusion The silicone membrane can be applied as an useful carrier for the keratinocytes transplantation.