双峰驼精清诱导排卵活性因子的HPLC分离纯化及生物活性测定

Purification of Ovulation-inducing Factors in the Seminal Plasma of Camel (Camelus Bactrianus) by HPLC and Capillary Electrophoresis and Analysis of their Biological Activity

利用C8 柱经HPLC对QHyperD柱分离的驼精清活性组分F3和F5进行分离纯化 ,大鼠垂体组织培养 ,RIA测定培养液中LH和FSH的浓度。实验结果表明 ,F3和F5经过HPLC纯化后 ,各得到 4个组分 ,分别以F3-1～F3- 4和F5 - 1～F5 - 4表示。其中F3- 3和F5 - 3可引起体外培养的大鼠垂体LH的释放显著增加 ,但均未引起FSH发生明显变化。用C18柱经HPLC或毛细管电泳对F3- 3进一步纯化 ,无论在HPLC或毛细管电泳仪中 ,F3- 3均呈现两个波峰 ,而且间隔非常靠近 ,说明F3-

The ovulation inducing factors were isolated from the biological fractions F3 and F5 by HPLC using C 8 column.F3 and F5 were separated into 4 fractions respectively,both the third fractions (F3-3 and F5-3) had the biological potential to stimulate the release of LH in the rat pituitary tissue culture media in vitro .F3 3 and F5 3 could increase the concentration of LH in the culture media significantly,and LH concentrations before and after addition of fraction were 16 02±2 40 and 32 14±4 56 mIU/ml,and for F3-3 3 37±0 51 and 7 76±1 16 mIU/ml for F3-3,respectively,however F3-3 and F5-3 could not stimulate the secretion of FSH in vitro .Finally,we tried to purify the ovulation inducing factors from F3-3 by HPLC using C 18 and capillary electrophoresis but could not success due to the similarity of molecular characters.From the above results,it can be concluded that the ovulation inducing factors of seminal plasma of camel is composed of several parts which have very similar molecular characters.