一氧化氮供体硝普钠降低肉鸡肺动脉压试验研究

Study on Reduction of Pulmonary Arterial Pressure by Sodium Nitroprusside in Broilers

AA肉鸡 10 0羽 ,随机等分为对照组、试验组 ,试验组肉鸡自 8日龄起给予含 0 2 %的钠离子的饮水以诱发肉鸡肺动脉高压综合征 ,按常规方法饲养。并通过右心导管法观察血管内注入硝普纳 (SodiumNitroprusside ,SNP)前后两组肉鸡肺动脉平均压 (Meanpulmonaryarterialpressure,mPAP)变化 ,与此同时 ,还观察一氧化氮 (NitricOx ide,NO)与肉鸡肺动脉压间的关系。结果发现试验组肉鸡mPAP高于对照组 (P <0 0 1) ,注入SNP后试验组和对照组肉鸡血浆NO水平都显著升高 (P <0 0 1) ,而mPAP都显著降低 (P <0 0 1) ,试验组肉鸡mPAP降压幅度大于对照组 (P <0 0 5 )。表明SNP可通过释放NO降低肉鸡mPAP。

broiler chickens were divided into control and experimental groups evenly.Broilers in experimental group were treated with additional 0 2% Na + (from NaCl) in drinking water from 8 to 49 days of age.Before and after Sodium Nitroprusside(SNP) infusion,values of mean pulmonary arterial pressure(mPAP) and plasma Nitric Oxide(NO) were evaluated by using a right cardiac catheterization procedure in 30 broilers of two groups.It was found that experimental group had a higher mPAP(P<0 01) than the control group.Reduction of mPAP from 25 96±2 96 mmHg to 18 39±3 69 mmHg(P<0 01),and increase of plasma NO concentration from 16 70±3 41 umol/L to 23 44±2 70 umol/L(P<0 01) in experimental group were found by SNP intravenously infusion,in the meantime in control group mPAP reduction from 17 59±1 05 mmHg to 13 89±0 36 mmHg(P<0 01) and plasma NO increase from 13 64±1 40 umol/L to 24 89±3 33 umol/L(P<0 01).It was concluded SNP could reduce broiler pulmonary arterial pressure by releasing NO.Further study on the role of NO in broiler pulmonary arterial hypertension syndrome could be applied in explanation of pathogenesis of the disease.