甘肃省进口肉牛冷冻胚胎移植的研究

Transplantation of Imported Frozen Beef Embryo in Gansu Province

1 999年 5月至 2 0 0 0年 1 2月在甘肃省武威市、平凉市、崇信县、灵台县开展了进口肉牛冷冻胚胎移植工作。经过对 386头当地黄牛母牛及杂交母牛进行了诱导同期发情 ,结果获得84.9%的同期发情率和 77.1 %的黄体合格率。对于黄体合格的 2 5 3头母牛进行了进口肉牛冷冻胚胎移植 ,获移植妊娠率 5 0 .9% ,其中 45头受体牛由甘肃省家畜繁育中心技术人员移植 ,获得 5 1 .1 %的移植妊娠率。对同一批次经产牛与青年牛移植妊娠结果、常规法与一步法解冻胚胎移植妊娠结果进行了对比分析 ,结果显示 ,经产牛移植妊娠率 ( 37.5 % )显著低于青年牛( 5 6.6% ) ,常规胚胎解冻法与一步细管解冻法解冻胚胎 ,移植妊娠率差异显著 ( 4 1 .6% ,69.5 % ,P<0 .0 5 ) ,后者较高。

Transplantation of imported frozen beef embryo was carried out in Wuwei, Pingliang, Congxin and Lingtai county in Gansu province from May of 1999 to December of 2000. 386 cows of local yellow or their crossbred progeny were treated with induced synchronization estrus, the percentage of synchronization and eligible lutein was 84.9 percent and 77.1 percent, respectively. 253 cows with eligible lutein were transplanted with imported frozen beef embryo, the gestation percentage reached 50.9 percent, among which 45 cows were operated by our technicians and got a 51.1 percent gestation percentage. Results also showed that, on the same conditions, the gestation percentage of postpartum cows was significantly lower than that of the heifers and one-step thawing procedure was better than traditional procedure.