碱性成纤维细胞生长因子对增生性瘢痕的作用

Effects of bFGF on hypertrophic scar in a nude mouse model

目的探讨碱性成纤维性细胞生长因子 (bFGF)对移植至裸鼠皮下的人增生性瘢痕的作用。方法将烧伤患者手术切除的增生性瘢痕移植到裸鼠背部皮下 ,动物随机分为 5组 :对照组、bFGF组、胶原酶组、bFGF +胶原酶组和玻璃酸钠组。瘢痕块移植后 2周分别用上述不同药物局部注射至瘢痕内共 3次 ,每次间隔 3d ,后观察疤痕大小、硬度的变化 ,第 1次注射药物后 3周活检作组织学检查。结果bFGF组瘢痕缩至原瘢痕大小的 1/ 2～ 1/ 3且软 ,能使粗大密集的胶原束变松散成团 ,其结构大部分消失。bFGF +胶原酶组所移植的瘢痕组织块有 2 / 3消失。结论增生性瘢痕局部应用bFGF可以降解瘢痕胶原 。

ObjectiveTo investigate the effects of bFGF on human hypertrophic scar in a nude mouse model. MethodsHuman hypertrophic scars excised in operation for burned patiens were grafted onto the back of nude mice subcutaneously and the mice were randomly divided into: control group, collagenase group, bFGF group, and bFGF plus collagenase group. Two weeks after grafting, preparations were injected into the scars for three times. The size, hardness, and morphological changes of the scars were examined. Biopsies were done 3 weeks after first injection and the histological changes were examined. ResultsIt was found that in bFGF group the size of the grafted scars reduced to 1/2～1/3 of their original size, the majority of the coarse and dense scar collagen bundles resolved and became soft loosed lump. In bFGF plus collagenase group, two thirds of the grafted scar disappeared. ConclusionTopical injection of bFGF into hypertrophic scar degrades scar collagen.