中国北方汉族人细胞色素P4501 A1基因MspⅠ多态性的研究

Study in genetic polymorphisms of cytochrome P4501A1 among Hans in North China

目的 探讨中国北方汉族人细胞色素P(cytochromeP ,CYP) 4 5 0 1A1基因MspⅠ多态性。方法 用聚合酶链反应 限制性片段长度多态性 (PCR RFLP)技术 ,分析了 172名北方汉族正常健康成人CYP1A1基因 3′端限制性内切酶MspⅠ位点的3种基因型 (A、B、C)的分布频率。结果 MspⅠ等位基因m1、m2分别占 6 0 8%、39 2 %。MspⅠ基因型A占 34 9% ,基因型B占 5 1 7% ,基因型C占 13 4 %。结论 本研究结果提示中国北方汉族人解毒酶CYP1A1基因存在MspⅠ多态性。

Objective To assess the MspⅠ polymorphisms of CYP1A1 among Hans in North China.Methods Using polymerase chain reaction restriction fragment length polymorphism(PCR RFLP),three genotypes A,B and C in 3′ flanking region of CYP1A1 were studied in 172 Hans in North China.Results The frequencies of genotype A,B and C were 34.9%,51.7% and 13.4%,respecitively,while allele m1 and m2 were 60.8% and 39.2%,respectively.Conclusion Our results suggest that CYP1A1 has Msp Ⅰ polymorphisms among Hans in North China.