大鼠局灶性脑缺血后远隔区域脑损伤及药物干预的研究

Study of brain injury in the long distance region and medicine intervene after partial ischemic attack in rat

目的 观察大鼠局灶性脑缺血后急性期两侧额叶、丘脑、小脑、下丘脑局部脑血流量 (rCBF)和细胞形态学的变化 ;评估川芎嗪对上述变化的干预作用。方法 采用线栓法建立大鼠一侧大脑中动脉闭塞 (middlecerebralarteryocclusion ,MCAO)模型 ;用氢清除法测定两侧额叶、丘脑、小脑、下丘脑在MCAO后 1、3、6和 2 4h的rCBF ;用HE染色和TUNEL法观察两侧额叶、丘脑、小脑MCAO后 6h、2 4h、1周细胞形态学变化。结果 正常对照组各相应部位rCBF无显著差异 (P >0 0 5 ) ;一侧MCAO后 ,两侧额叶皮层、丘脑、下丘脑、对侧小脑半球rCBF下降 ,MCAO后 1h达最低水平 (P <0 0 5或 P <0 0 1) ,随时间延长 ,rCBF逐渐增加 ,除对侧小脑、下丘脑外 ,其余部位rCBF至MCAO后 2 4h基本恢复正常 ;而川芎嗪可明显抑制上述rCBF的下降程度 (P <0 0 5 )。同侧额叶、丘脑在MCAO后 6h开始出现TUNEL阳性细胞 ,2 4h后明显增加 ,至 1周阳性细胞减少 (P <0 0 5 ) ,而川芎嗪可明显抑制上述部位凋亡细胞的产生。结论 局灶性脑缺血后可引起远隔区域有关脑组织rCBF下降 ,并进一步引发细胞凋亡。

Objective To observe the change of regional cerebral blood flow(rCBF) and morphology of bilateral frontal lobe,thalamus,cerebellum,hypothalamus of rats after partial ischemic attack.Evaluated the effect of Chanqiongzhen on above mentioned.Methods Models of middle cerebral artery occlusion(MCAO)were established by using thread bolt.The rCBF mophology changes were surveyed by HE staining and TUNEL method.Results There was no remarkable difference observed between every corresponding position in normal group(P<0 05)and production of cell apoptosis.Conclusion Partial ischemic attack cause cell apoptosis,the Chuanqiongzhen can inhibite change above mentioned remarably.