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分子生物技术在蜜蜂育种研究上的应用 被引量：1

Application of molecular biotechnology to honeybee's breeding
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摘要 综述了应用微卫星 DNA技术、线粒体 DNA(mt DNA)技术、随机扩增多态性 DNA(RAPD)、聚合酶链式反应 (PCR)和 DNA指纹等分子生物技术所进行的蜜蜂育种研究现状 .其应用主要包括估计蜂群内亚家系(父系 )的组成数目和分析工蜂间的遗传相关 ,推断蜂王的多雄水平和鉴定蜜蜂种性 ,构建连锁图和区别数量性状位点 ,鉴定基因型和区别全胞。 This paper reviewed the advances in honeybee breeding involving to estimate the numbers of subfamilies (paternal line) in a colony and analyze the genetic correlation among workers; guess the polyandry level of queens and identify the purity of a subspecies; build a linkage map and discriminate loci of quantitative traits; genotype and distinguish full sister from semi sisters; analyze the trend of sperm to inseminate an egg and so on by means of such molecular biotech as microsatellites DNA, mitochondrial DNA, random amplified polymorphismic DNA, polymerase chain reaction and DNA fingerprint.
作者 王丽华 苏松坤 邵瑞宜
机构地区 福建农林大学蜂学学院 浙江大学动科院蚕蜂系
出处 《福建农业大学学报》 CSCD 北大核心 2001年第4期518-523,共6页 Journal of Fujian Agricultural University
关键词 蜜蜂 育种 生物技术 分子生物技术 honeybee breed biotechnology
分类号 S892.6 [农业科学—特种经济动物饲养]
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参考文献28

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  • 2[2]GARNERY L, FRANCK P, BAUDRY E, et al. Genetic diversity of the west European honeybee (Apis mellifera mellifera and A.m.iberica) Ⅱ[J]. Microsatellite loci Genetics, Selection, Evolution, 1998,30(Supplement):49-74.
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同被引文献16

  • 1M. K. Fondrk,R. E. Page,G. J. Hunt.Paternity analysis of worker honeybees using random amplified polymorphic DNA[J]. Naturwissenschaften . 1993 (5)
  • 2Greg J. Hunt,Robert E. Page.Patterns of inheritance with RAPD molecular markers reveal novel types of polymorphism in the honey bee[J]. Theoretical and Applied Genetics . 1992 (1)
  • 3GREG J H,ROBERT E,PAGE J R,et al.Major quantitative trait loci affecting honey bee foraging behavior. Genetics . 1995
  • 4Greg J,Hunt,Novoa E,Guzman M K,Fondrk,et al.Quantitative trait loci for honey bee stinging behavior and body size. Genetics . 1998
  • 5Beye M,Neumann P,Schmitzova J,et al.A simple,non-radioactive DNA fingerprinting method for identifying patrilines in honeybee colonies. Apidologie . 1998
  • 6FONDRK M K,ROBERT E,PAGE J R,et al.Paternity analysis of worker honeybees using random amplified polymorphic DNA. Naturwissenschaften . 1993
  • 7GREG J,HUNT RE,PAGE Jr.Patterns of inheritance of RAPD molecalar markers in the honey bee reveal novel types of polymorphism. Theoretical and Applied Genetics . 1992
  • 8GREG J Hunt,ROBERT E,PAGE,et al.Linkage map of the honey bee, Apis mellifera, based on RAPD markers. Genetics . 1995
  • 9蒋滢,沈伟华,汪成富,黄超群,张大隆,薛运波.制备K700bp成探针与美意和平湖两品系西蜂RAPD-PCR扩增产物及它们基因组DNA分子杂交的研究[J].中国养蜂,2000,51(2):6-7. 被引量：8
  • 10张雅娟,蒋滢,王尉平,汪成富,葛凤晨,张大隆.W316bp探针鉴定高、低产王浆西蜂DNA分子特异标记的研究[J].中国养蜂,2001,52(2):6-8. 被引量：10

引证文献1

福建农业大学学报
2001年 第4期

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