不同处理菌糠细胞壁成分及有机物瘤胃降解特性

Ruminal degradability of organic material and cell wall constituent of waste material from fungal culture by different treatments in nylon bags for cattle

对经化学、生物及复合 3种处理的菌糠的细胞壁成分进行分析 ,研究不同处理对提高菌糠饲用价值的效果 .选用 4头装有永久瘤胃瘘管的杂交黄牛 ,按 4× 4拉丁方设计 ,采用尼龙袋法研究化学、生物及复合等 3种处理菌糠 OM瘤胃降解特性 .结果表明 :(1)香菇、茶树菇及金针菇等 3种菌糠经化学处理后 ,NDF、ADF、ADL均有不同程度降低 ,其中以 Na OH处理效果最佳 ,NDF、ADF、ADL分别下降 5 .71% - 9.92 %、7.4 3% -13.36%及 10 .5 5 % - 2 0 .4 7% ;经筛选 ,Y4、Y5及 Y8种真菌能显著降低香菇菌糠 NDF、ADF、CEL、HC及 ADL含量 ,其中以 Y8菌种效果最佳 ;Na OH和生物 (Y4 )复合处理对改善香菇菌糠细胞壁成分的效果优于单独的Na OH-生物 (Y4 )处理 .(2 )香菇菌糠经 Na OH、氨化 (尿素 )及氨化 (尿素 ) +Na OH处理后 ,OM的动态降解率(P)分别提高 19.60 %、13.16%、2 1.0 2 % (p<0 .0 5 ) ;生物处理中以 Y4、Y5及 Y8三种真菌的处理效果最佳 ,菌糠 OM动态降解率 (p)分别提高 31.65 % (p<0 .0 5 )、4 7.69% (p<0 .0 1)及 5 5 .82 % (p<0 .0 1) ,其中 Y8和 Y5菌种的效果又优于 Y4 (p<0 .0 5 ) ;Na OH-生物 (Y4 )复合处理效果优于单独的 Na OH或生物 (Y4 )

Cell wall constituents(CWC) of the waste material from fungal culture(WMFC) by different treatments were assayed for studying the effect of different treatments such as chemical, microorganism, combination of chemical and microorganism on improvement of the feeding value of WMFC. Four hybrid yellow cattle fitted with rumen cannulas were selected to study degradability of organism material(OM) of WMFC by above different treatments using the method of rumen by 4×4 Latin square design. The results were as follows: (1) The three kinds of WMFC such as neutral detergent fiber(NDF), acid detergent fiber(ADF) and acid detergent lignin(ADL) decreased by chemical treatment, the effect of NaOH treatment was the best in all chemical treatments. NDF, ADF, ADL decreased by 5.71%-9.92%, 7.43%-13.36% and 10.55%-20.47%, respectively; three kinds of white rot fungi (Y4, Y5 and Y8) decreased NDF, ADF, CEL, HC and ADL of waste material from Lentinus edodes(WMLE) significantly, among these treatments the effect of Y8 was the best, and the effect of combination treatment of the NaOH and microorganism on the improvement of the CWC was better than that of single chemical or microorganism treatment. (2) The effective degradability of OM of WMLE by the three kinds of chemical treatment(NaOH, urea and urea+NaOH) increased by 19.60%, 13.16% and 21.02% respectively( p <0.05). The effects of Y4, Y5 and Y8 were best in microorganism treatments, their effective degradability of OM of WMLE increased by 31.65%,( p <0.05), 47.69%( p <0.01) and 55.82%( p <0.01), respectively. The effects of Y5 and Y8 were better than those of Y4. The Effect of combination treatment of NaOH and microorganism was better than that of single chemical or microorganism treatment on improvement of CWC of WMLE and CWC.