腮腺肿瘤及瘤旁腺泡p16、p21和DNA定量研究

A qualititative study on p16 mutiple tumor supp ressor gene, rasoncogene p21 and DNA content in parotid tumor and its′ contiguous acini

目的 探索腮腺肿瘤切除后瘤旁腺泡生物学特性与肿瘤复发及恶变的关系。方法 应用免疫组织化学、流式细胞术对 68例腮腺肿瘤及瘤旁腺泡p16、p2 1的表达和DNA定量进行了分析。结果 良恶性肿瘤p16、p2 1蛋白表达阳性目标值明显高于正常腺泡 (P <0 0 1) ,表达区域主要位于腺管样或肌上皮团块区的细胞浆中 ,部分肿瘤及瘤旁腺泡分别为强阳性和弱性表达 ,复发性腮腺深叶包膜不完整肿瘤阳性目标值高于原发性腮腺浅叶包膜完整的肿瘤 (P <0 0 5 )。DI、S %和PI指数良恶性肿瘤及瘤旁腺泡均高于正常腺泡 (P <0 0 5 )。结论 当p16多肿瘤抑制基因丢失 ,p2 1癌基因突变时 ,腮腺肿瘤瘤旁腺泡在术后可能处于一种“准新生物”状态 ,具有较强的增殖能力 ,它们在肿瘤复发 ,恶变中具有潜在的趋势。

Objective To determine the biologic characteristics of tumor′s contiguous acini and the r elationship of recurrence or malignant transformation. Methods With the use of immunohistochemistry and flow cytometry , the features of p16, p21 and DNA expression in 68 tumors and their contiguous acini were studied. Results The expression of p16 and p21 gene wrer found in tumors that the possitive unit (PU) was higher than normal acini (P<0.01). Th e areas of expression were mainly located in cytoplasm of gland duct cells. Stro ng possitives or weak possitives could be observed in the tissues of malignant tumors and their contiguous acini respectively, as well as the PU in the deep lo be of recurrent parotid tumors in which the capsule was incomplete was higher th an that in the superficial lobe of primary parotid tumors with complete capsule (P<0.05). Futhermoer the levels of DI, S% and PI were higher in parotid tumors and its contiguous acini than normal acini (P<0.05). Conclusions When p16 multiple tumor suppressor gene is lost and oncogene p21 is mutated, the tumor′s contiguous acini maybe in the condition o f 'paraneoplasm' after parotid tumor removal, and it has a strong ability of p reliferation, which play an important role in recurrent or malignant change of t he parotid tumor.