转化生长因子β对舌癌细胞系Tca83生长的影响

The role of TGF-β on Tca83--a tongue squamous cell carcinoma cell line

目的 观察转化生长因子 β(TGF β)对于Tca83细胞生长的作用。 方法 舌癌细胞常规培养 ,以MTT检测细胞增殖 ,免疫组化、原位杂交检测TGF β及其受体TβRⅠ的表达。 结果 加入TGF β 96h后 ,大多数实验组细胞的生长受到抑制。这种抑制作用与TGF β的浓度有关 ,较低浓度的TGF β( 0 2、1 0、5 0和 12 5 μg/L ,其A值分别为 0 73 5、0 75 8、0 760和 0 75 7)能够抑制Tca83细胞的生长 (与对照组A值 0 911比较 ,P <0 0 1) ,而高浓度 ( 2 5 0 μg/L)的TGF β反而失去了这种抑制作用。Tca83细胞系中可检出TGF β1、TGF β受体 (TβR)Ⅰ的蛋白及mRNA的阳性表达、TβRⅡ蛋白的阳性表达。结论 TGF β能抑制Tca83细胞的生长。Tca83细胞内的TβRⅠ及TβRⅡ表达使这种抑制作用成为可能。

Objective To investigate the role of TGF-β on the growth of Tca83 cells. Methods The cell culture, MTT detection, immunohistochemistry a nd in situ hybridization detection methods were used. ResultsAfter 96 h, compared with the control, most groups wi th low concentrations of TGF-β (0.2, 1.0, 5.0, 12.5 μg/L) showed gro wth inhibition, while the group with high concentration of TGF-β (25.0 μg/L) sho wed no growth inhibition. Positive stain of TGF-β1, TβRⅠ mRNA and TGF-β1, TβRⅠ,TβRⅡ protein could be detected in Tca83 cells. Conclusions Tca83 cell line has the potentiality to secret TGF -β1. Autocrine and paracrine of TGF-β1 could regulate cancer cell′s prolife rat ion and differentiation directly. Since the ligand-receptor conduction in Tca83 cell line is intact, external TGF-β1 could combine with the TβRⅠ and TβRⅡ receptor and exert its inhibitory effect on Tca83 cell line.