中草药抗致龋菌的实验研究

Experimental study of bacteriostatic activity of Chinese herbal medicines on primary cariogenic bacteria in vitro

目的 筛选出具有抗致龋菌的中草药 ,并检测其最小抑菌、杀菌浓度。方法 对各种实验用中草药分别采用水、醇两种方法提取有效成分 ,从黄连中提纯盐酸小檗碱 ,通过纸片扩散法筛选出具有抗致龋菌的中草药 ,用液体稀释方法检测最小抑菌及最小杀菌浓度。结果 对变形链球菌Ingbritt株的最小杀菌浓度 ,厚朴、黄连、五倍子、大黄醇提取物、黄连水提取物和小檗碱分别是 0 4 88、1 95 0、7 80 0、3 1 2 5 0、7 80 0及 0 62 6g/L ;对茸毛链球菌 6715株的最小杀菌浓度 ,厚朴、黄连、大黄醇提取物、黄连水提取物和小檗碱分别是 0 4 88、3 90 0、3 1 2 5 0、1 95 0及 0 62 5g/L ;对粘性放线菌ATCC192 4 6株的最小杀菌浓度 ,黄连、大黄醇提取物、黄连水提取物和小檗碱分别是 3 90 0、3 1 2 5 0、3 90 0及 1 2 5 0g/L。结论 厚朴、黄连、大黄醇提取物、盐酸小檗碱、黄连及大黄水提取物对致龋菌有较强的抑菌或杀菌作用 ,厚朴醇提取物的杀菌效果最强。

Objective To screen some Chinese herbal medicines for their in hibitory activity on cariogenic bacteria, and investigate their active ingredien ts, and measure their minimum inhibitory concentration (MIC), minimum bactericid al concentration (MBC). Methods Active components were isolat ed from every test ed Chinese herbal medicine by means of aqueous extraction and ethanolic extracti on. Berberine was purified from Coptis chinensis Fra. Disk agar diffusion meth od was employed in screening herbs with inhibiting effect on cariogenic bacteria. MIC and MBC were determined by broth dilution method. Results Against Streptoco ccus mutans Ingbritt, MBCs of Magnolia officinalis ethanolic extract, Berberin e, Coptis chinensis Fra aqueous extract and Coptis chinensis Fra ethanolic extract were 0.488, 0.625, 7.800 and 1.950 g/L respectively. Against Streptococcu s sobr inus 6715, MBCs of Magnolia extract, Coptis chinensis Fra ethanolic extract, R hu s chinensis Mill ethanolic extract and Phellodendron chinen ethanolic extract we re 0.488, 0.625, 1.950, 3.900, 3.900 and 3.900 g/L respectively. Against Actinom yces viscosus ATCC 19246, MBCs of Berberine, Coptis chinensis Fra aqueous extr ac t, Coptis chinensis Fra ethanolic extract, Rheum palmatum L aqueous extract and Rheum palmatum L ethanolic extract were 1.250, 3.900, 3.900, 15.600 and 31. 250 g/L respectively. Conclusions Magnolia officinalis, Coptis chi nensis Fran, Rheum palmatum L aqueous extracts exhibit strong inhibition on cari ogenic bacteria. Magnolia officinalis ethanolic extract has the strongest bacter icidal effects on Streptococcus mutans and Streptococcus sobrinus.