Autotaxin基因、CD44V6分子、DNA含量与胃癌淋巴结转移的关系及三者相关性研究

Relationship Between Autotaxin Gene,CD44V6 Molecule,DNA Content and Lymph Node Metastasis of Gastric Carcinoma

目的 检测胃癌组织中自分泌运动因子 (ATX)、CD44V 6的表达及DNA含量 ,观察它们与胃癌淋巴结转移的关系。方法 对 36例胃癌组织 ,分别采用半定量逆转录—聚合酶链反应检测ATXmRNA表达 ,流式细胞术检测CD 44V 6分子表达及DNA含量 ,并对三者进行相关性分析。结果 胃癌组织中ATX基因表达率为 10 0 .0 0 % ,其表达值为 6 4.34%± 15 .15 % ;CD44V 6分子表达率为 6 9.44% ,表达值为 3.0 6 %± 1.14 % ;细胞核DNA含量指数为 1.39± 0 .2 3。ATX、CD 44V 6、DNA含量三者均与胃癌淋巴结转移有关 ,且三者之间均呈正相关。结论 同时检测ATX和CD 44V 6的表达及细胞核DNA含量 ,对判断胃癌的浸润转移及其预后具有指导意义。

Objective To survey Autotaxin(ATX) and CD44V6 expression and DNA content in gastric carcinoma tissues and the relationship between three factors and lymph node metastasis of gastric carcinoma.Methods Thirty-six gastric carcinoma specimens were obtained from surgery.Semi-quantitative reverse transcription polymerase chain reaction was used to detect mRNA expression of ATX;on the other hand,CD44V6 molecule expression and DNA content were studied by flow cytometry.Results The expression rate of ATX gene was 100.00% in gastric carcinoma,the mean expression level was 64.34%±15.15%.The expression rate of CD44V6 molecule was 69.44% in gastric carcinoma,the mean expression level was 3.06%±1.14%.DNA relative content was 1.39±0.23 in gastric carcinoma.ATX,CD44V6 and DNA content were all relative to lymph node metastasis of gastric carcinoma.There was a positive correlation among the three factors.Conclusion Combined examination of ATX and CD44v6 expression and DNA content,has directive significance to judge invasion,metastasis and prognosis of gastric carcinoma.