茶多酚对顺铂所致肾损伤的作用及其可能机制探讨

Effects of tea polyphenols on renal damage caused by cis-platinum and its possible mechanism

目的 研究茶多酚 (TP)对顺铂 (DDP)所致肾毒性作用的保护作用及其可能机制。方法 小鼠随机分为4组 :对照组、DDP组、DDP +高剂量TP组、DDP +低剂量TP组 ,分别给药处理后 ,观察血清中尿素氮 (BUN)、肌酐 (Cr)变化情况 ,测定肾组织匀浆中超氧化物歧化酶 (SOD)、谷胱甘肽过氧化物酶 (GSH Px)的活性。结果 DDP组的血清中BUN及Cr值上升与对照组比较差异有显著性 (P <0 0 5 ) ,加入高剂量TP以后血清中BUN及Cr值低于DDP组 (P <0 0 5 )。给予DDP后SOD、GSH Px活性降低与对照组比较差异有显著性 ;DDP +高剂量TP组SOD及GSH Px值高于DDP组 (P <0 0 5 )。结论 TP在一定程度上缓解DDP所致的肾损伤 ,其可能机制可能与抗氧化功能有关。

Objectives To study the protective effects of tea polyphenols(TP)on renal damage caused by cis-platinum(DDP)and its possible mechanism.Methods Mice were divided into four groups randomly,i.e.,control group,DDP group,DDP plus high-dose TP group and DDP plus low-dose TP.After treatment,changes in their serum level of urea nitrogen(BUN)and creatinine(Cr)were observed and activities of superoxide dismutase(SOD)and glutathione peroxidase(GSH-Px)were determined.Results Serum levels of BUN and Cr increased in the DDP group,as compared with the controls,with a significant difference(P<0.05).Levels of BUN and Cr in the DDP plus high-dose TP group were significantly lower than those in the DDP group(P<0.05).Activities of SOD and GSH-Px in the DDP group were significantly lower than those in the controls.Activities of SOD and GSH-Px in the DDP plus high-dose TP group were significantly higher than those in the DDP group(P<0.05).Conclusion TP could alleviate renal damage caused by cis-platinum to certain extent,with a possible mechanism of antioxidation.