中华绒螯蟹线粒体COⅡ基因全序列测定

The Complete Sequence of the Mitochondrial COⅡ Gene of Eriocheir japonica sinensis (Crustacea: Malacostraca: Dacapoda)

通过克隆测序方法 ,报道了中华绒螯蟹 (Eriocheirjaponicasinensis)线粒体细胞色素氧化酶亚基Ⅱ (COⅡ )基因全序列 .与已知甲壳动物线粒体同源序列的比较显示 ,绒螯蟹COⅡ基因的核苷酸组成及其编码的氨基酸组成与软甲类甲壳动物最相近似 .其序列组成与密码子使用对A、T核苷酸没有明显的偏倚 .对中华绒螯蟹COⅡ全序列及其序列特征的研究 。

The complete sequence of the mitochondrial cytochrome oxidase subunit Ⅱ (COⅡ) gene of malacostracan crustacean Eriocheir japonica sinensis has been determined.Comparisons of this sequence with the homologous sequences from the other six crustaceans and one insect indicate that the nt composition and aa composition of E.j.sinensis are more similar to other malacostracans than they are to the branchiopod crustaceans.The sequence of E.j.sinensis is less A+T-rich than the counterpart of insect and the pattern of codon usage is less biased.