鹅副粘病毒的分离与鉴定

Isolation and Identification of the Goose's Paramyxovirus

采用鸡胚接种法从安徽凤阳某患病鹅群中分离到一株病毒。经HA与HI试验、血清中和接种鸡胚试验、病毒回归试验确认为鹅副粘病毒 ,并命名为WF0 0 G株。参照新城疫病毒毒力判定的标准及其方法 ,测定该分离株的鸡胚最小致死量平均死亡时间 (MDT)、1日龄鸡脑接种致病指数 (ICPI)和 6周龄鸡静脉内接种致病指数 (IVPI)分别为 4 4 8h、1 81和 2 32 ,结果表明该分离株具有与新城疫病毒 (NDV)速发型相类似的毒力 ,属强毒力毒株。

One strain virus was isolated from affected Gooses livers spleens and pancreas in Fengyang of Anhui using chicken embryos and characterized as the Gooses paramyxovirus named WF-\ 00 G strain by hemagglutination (HA) hemagglutination-inhibition (HI)test inoculation of embryos after neutralization using particularsera and challenge with 3-week old geese and 3-week specific pathogen free (SPF)chickens the biologicai characterisites of the virus was derermined by measuringthe mean death time of chicken embryos(MDT)the intracerebral pathogenicity ingex in day-old chicks (ICPI) and the intravenous pathgenicity index in 6-week-old chickens (IVPI) at the virulence of Newcastle disease vius (NDV) the MDT ICPI and IVPI and IVPI were 44.8h 1.81 and 2.32 respectively. The results indicated that the isolate was high virulence like velogenic Newcastle disease virus (NDV)