低剂量X射线全身照射后小鼠IL-10和IL-12的反向变化

CHANGES IN OPPOSITE DIRECTIONS FOR THE EXPRESSION OF IL-10 AND IL-12 AFTER LOW DOSE IRRADIATION

通过检测低剂量辐射 (LDR)对小鼠脾淋巴细胞中IL - 10以及腹腔巨噬细胞中IL - 12的影响 ,研究LDR辐射免疫效应的机制。采用Northernblot检测了 75mGyX射线全身照射后 ,IL - 10、IL - 12mRNA水平的变化 ,同时分别通过流式细胞术和ELISA检测了IL - 10、IL - 12蛋白水平的变化。(1)Northernblot检测表明 ,75mGyX射线全身照射后脾细胞中IL - 10的mRNA转录水平从照射后 1h即降低 ,并维持较低水平 ,一直到 4 8h开始恢复 ,达到假照射水平的 86 .2 % ;巨噬细胞中IL- 12两亚基的mRNA水平的变化则表现为 ,照射后 1hp35及p4 0亚基均迅速升高分别达到假照射组的 131%和 192 %。而后 ,p35开始回降 ,直至照射后 16 - 4 8h恢复正常 ,p4 0亚基从照射后 1-4 8h总体表现为升高。(2 )对蛋白产物检测结果表明 ,脾细胞IL - 10合成在照射后 2h开始即呈时间依赖性降低 ,至 4 8h仍无恢复迹象 (p <0 .0 1) ,双抗体夹心酶联免疫吸附法 (ELISA)检测表明 ,巨噬细胞分泌IL - 12明显增高。LDR引起IL - 10和IL -

To observe the effects of LDR on the synthesis of IL-10 in splenocytes and the secretion of IL-12 by peritoneal macrophages. Northern blot and flow cytometry were adopted to detect the changes of IL-10 at mRNA and protein level, respectively. Northern blot and ELISA were used respectively to examine the changes of IL-12p35/p40 mRNA and IL-12p70 protein levels. In splenocytes IL-10 mRNA decreased significantly, while both IL-12 p35 and p40 subunits in macrophages increased after WBI with 75 mGy X rays. Meanwhile, IL-10 synthesis in splenocytes decreased beginning from 4h after exposure and remaining at low level up to 48h, and IL-12 secretion by macrophages was found to take an opposite direction. WBI with 75mGy X rays may suppress IL-10 both at the mRNA level and protein level and stimulate IL-12 expression simultaneously, and this would lead to a shift of the immune response in favour of T H 1 differentiation.