摘要
Ig Y是从鸡蛋黄中提取的免疫球蛋白 ,不但具有高免疫活性 ,而且不受类风湿因子、血清补体等干扰 ,在免疫学试验中具有优越性 .用纯化的弓形虫虫体抗原免疫白莱杭鸡获得特异性的Ig Y抗体 ,用辣根过氧化物酶标记后与抗人 Ig M单抗配伍建立捕获法酶免疫试验 ,与同类试剂对照 ,平行检测 192份临床血样 ,检测结果二者吻合 ,表明该 Ig Y具有良好的特异性和敏感性 .
IgY was an immunoglobulin extracted from egg yolk. It did not react with rheumatoid factor, mammalian complement and mammalian Fc receptors etc in enzyme-linked immunosorbent assay(ELISA). The purified Toxo antigen was used to immuning white leghorn hens, and the anti-Toxo IgY antibody was received. The specific IgY was labelled by horseradish peroxidase, and matched with anti-human IgM to establish ELISA and 192 clinical sera were detected. The results showed that the control group and the detected one were identical.
出处
《兰州大学学报（自然科学版）》
CAS
CSCD
北大核心
2001年第6期76-79,共4页
Journal of Lanzhou University(Natural Sciences)
关键词
IGY
弓形虫
酶联免疫吸附试验
血清
IgY
toxoplasma gondii
enzyme-linked immunosorbent assay(ELISA)
