细胞色素C_(551)在ITO电极上的直接电化学

Direct Electrochemistry of Cytochrome C_(551) from Pseudomonas Aeruginosa at ITO Electrodes

在不加任何电子转移促进剂的条件下，用循环伏安法（ＣＶ）和微分脉冲伏安法（ＤＰＶ）观察到细胞色素Ｃ５５１在ＩＴＯ导电玻璃上的直接电化学行为．结果显示电极反应过程为准可逆性质．计算得到细胞色素Ｃ５５１的扩散系数、式电位和异相电子转移标准速率常数，并对其在ＩＴＯ导电玻璃电极上的电子转移机制进行了初步分析．

The direct electrochemistry of cytochrome C-551 from pseudomonas aeruginosa has been investigated at indium-doped tin oxide(ITO) optically transparent electrodes without any surface promoters or mediators. The cyclic voltammograms show a quasi-reversible electrode reaction. Plots of peak reduction current against square root of scan rate are linear over the range 5 mV . s(-1) to 100 mV . s(-1). The diffusion coefficient of C-551 is calculated from the slope of the line. The rate constant for heterogeneous electron transfer is determined by measuring cyclic voltammetry peak separation as a function of scan rate.