凝血酶肽促进缺血创面愈合与皮瓣存活的实验研究

An experimental study of the effects of thrombin receptor activating peptide (TP508) on healing of ischemic wound and flap survival in rats.

目的 探讨凝血酶受体激活肽 (TP5 0 8)对促进缺血创面愈合与皮瓣存活的作用。方法SD大鼠 6 6只 ,制作部分缺血创面 (16只 )、完全缺血创面 (16只 )、正常创面 (18只 )及皮瓣 (16只 )模型 ,每一模型又分为TP5 0 8治疗组和等渗盐水对照组。术后第 3、7、10、14天 ,将创面或皮瓣坏死轮廓描记至醋酸纸上 ,输入计算机求出创面面积或坏死面积。 结果 术后 7d和 14d ,TP5 0 8组正常创面面积仅为对照组的 73 7%和 45 4%。术后 7d ,TP5 0 8组部分缺血创面面积为 (99 8± 30 7)mm2 ,而对照组为 (12 8 0± 43 4)mm2 。术后第 10天 ,TP5 0 8组完全缺血创面面积为 (2 93 0± 34 0 )mm2 ,对照组为 (35 2 4± 41 2 )mm2 。术后第 7天 ,TP5 0 8组皮瓣坏死面积为对照组皮瓣坏死面积的80 4% ,第 14天为 5 6 8%。 结论 TP5 0

Objective To investigate the effects of the thrombin receptor activating peptide (TP508) on healing of ischemic wound and flap survival in rats. Methods Sixty*Msix Sprague*MDawley rats were employed as the model. On the back of the rats, three kinds of wound and flap were made to establish four groups as follows: partial ischemic wound in 16, full ischemic wound in 16, routine wound in 18 and flap in 16 rats. Each group was further divided into TP508 treating group and isotonic saline control group. The total and necrotic areas of the wounds and flaps were daplicated on acetate papers and calculated with a computer on the 3rd, 7th, 10th and 14th post*Moperation days (PODs). Results In routine wounds, the ischemic wound area treated by TP508 was 73.7% and 45.4% in saline control groups on 7 and 14 (PODs), respectively. While in the flap model, the necrotic flap area treated by TP508 was 80.4% and 56.8% in control groups on 7 and 14 (PODs), respectively. Conclusion TP508 could accelerate healing of ischemic wound and improve flap survival in rats.