水稻黑条矮缩病毒基因组第九组分cDNA的克隆及序列分析

Molecular Cloning and Analysis os cDNA of Rice Black Streaked Dwarf Virus Genome Segment 9

从我国发病的玉米材料中提取水稻黑条矮缩病毒 ,抽提病毒RNA ,利用RT PCR等手段 ,获得了病毒基因组第九组分 (S9)cDNA克隆 .序列分析结果表明 :S9全长 190 0bp ,含有 2个不重叠的ORF ,编码蛋白的分子质量分别为 39.9,2 4.2ku ,与日本株S9核苷酸序列同源性为 89%,与意大利株MRDVS8同源性为 86 %.

Rice black streaked dwarf virus (RBSDV) and its RNA were extracted from infected maize plants grown in China. Virus genome segment 9 (S9) cDNA clone was obtained by RT-PCR.Sequence analysis results showed that RBSDV S9 (1 900 bp) contained two non-overlapping ORFs encoding 39.9 ku and 24.2 ku proteins respectively. The sequence homology was 89% between Chinese RBSDV S9 and Japanese RBSDV S9 and 86% between Chinese RBSDV S9 and Italian MRDV S8.