粘液表皮样癌p16、p53、nm23蛋白及其mRNA表达的临床病理意义

Detection of proteins and mRNA of p16, p53 and nm23 in mucoepidermoid carcinoma of salivary glands and their prognostic significance

目的 了解粘液表皮样癌中p16、p5 3、nm2 3蛋白及其mRNA表达的情况 ,探讨它们与粘液表皮样癌的病理形态学特征的关系。方法 用HE和组织化学方法对 4 1例粘液表皮样癌诊断 ,并按WHO标准分为高分化、低分化两组 ,用免疫组织化学SABC法和原位杂交方法 ,分别检测 4 1例粘液表皮样癌p16、p5 3、nm2 3蛋白及mRNA ,用统计学方法分析它们之间的关系。结果 粘液表皮样癌中低分化组、高分化组的p16蛋白失表达率分别是 62 .5 % ( 15 / 2 4 )和 2 9.4 ( 5 / 17) ,P =0 .0 3 7;p5 3蛋白的阳性率分别为 70 .8% ( 17/ 2 4 )和 2 3 .5 % ( 4 / 17) ,P =0 .0 0 3 ;nm2 3蛋白的阳性率分别为 3 7.5 % ( 9/ 2 4 )和 64.7% ( 11/ 17) ,P =0 .0 86。p16、p5 3、nm2 3mRNA的表达与病理分级无明显相关。结论 p16、p5 3蛋白表达与粘液表皮样癌的病理分级有显著相关性 ,可作为判断预后的辅助指标 ;p16、p5 3、nm2 3mR NA表达与病理分级无明显相关。

Objective To disclose the relationship between the expression of p16, p53 and nm23 and the significant morphologic characteristics in mucoepidermoid carcinoma. Methods The proteins and mRNA in paraffin blocks from 41 mucoepidermoid carcinoma patients were studied with immunohistochemical methods and in situ hybridization respectively. Results The negative expression of p16 was found in 62.5% (15/24) of the low differentiation grade and in 29.4% (5/17) of the high differentiation grade ( P =0.037). The positive p53 expression was detected in 70.8% (17/24) of the low grade neoplasms and in 23.5% (4/17) of the high grade ( P =0.003), while positive nm23 protein was present in 37.5% (9/24) and 64.7% (11/17) respectively ( P =0 086). However, no relationship was found between positive mRNA of p16, p53, nm23 and WHO pathologic grade. Conclusions There is a significant correlation between expression of p16 and p53 and WHO pathologic grade of mucoepidermoid carcinoma, which indicates clinical prognostic significance. No correlation is noticed between the pathologic grade and the expression of nm23 protein and mRNAs of p16, p53 and nm23.