鼻咽癌染色体3p14的精细等位基因缺失分析(英文)

Analysis of allelic loss on chromosome 3p14 in nasopharyngeal carcinoma

目的 进一步明确鼻咽癌染色体３ｐ１４区域等位基因杂合性丢失（ｌｏｓｓ ｏｆ ｈｅｔｅｒｏｚｙｇｏｓｉｔｙ，ＬＯＨ）的频率与共同缺失区范围，以便分离该区域内与鼻咽癌相关的候选抑瘤基因。方法 选择位于３ｐ１４的６个高密度微卫星多态标记，对３２例鼻咽癌组织进行ＬＯＨ分析。结果 ７１．８８％（２３／３２）的鼻咽癌在至少１个位点发生ＬＯＨ，丢失频率较高的３个位点是Ｄ３Ｓ１３１３（４６．４３％）、Ｄ３Ｓ１３００（５０．０％）和Ｄ３Ｓ１３１２（４４．４４％）。在存在丢失的２３例患者中，８例表现为一个连续的非随机的ＬＯＨ区域，其最小共同缺失区为Ｄ３Ｓ１３１３～Ｄ３Ｓ１３１２（约３．４个厘摩）。且该区域的缺失与鼻咽癌临床分期、ＥＢ病毒感染有明显关系。结论 鼻咽癌在３ｐ１４的最小共同缺失区位于Ｄ３Ｓ１３１３～Ｄ３Ｓ１３１２之间，该区域可能存在一个尚未克隆的与鼻咽癌发生发展密切相关的抑瘤基因。

Objective To further determine the frequency and common deletion region of allelic loss on chromosome 3p14 in nasopharyngeal carcinoma (NPC) in order to facilitate the isolation of the candidate tumor suppressor genes (TSGs) associated with NPC. Methods Six high dense microsatellite polymorphic markers on 3pl4 were selected to examine the loss of heterozygosity (LOH) in 32 NPCs. Results Of the 32 specimens, 23 (71.9%) showed LOH in at least one locus. High frequencies of LOH were observed at loci D3S1313 (46.4%), D3S1300 (50.0%), and D3S1312(44. 4%). Eight cases showed LOH in one continuous and nonrandom region between D3S1313 and D3S1312 (about 3.4 centimorgan). In addition, the LOH on 3p14 correlated with the tumor stage and Epstein-Barr virus (EBV) infection. Conclusion A putative TSG involved in nasopharyngeal carcinogenesis may be present at the smallest common deletion region between D3S1313 and D3S1312.