CpG DNA中ISS数量与免疫刺激效果间的关系初探

Preliminary study on the relationship between the immunostimulatory effects and the number of ISS in CpG DNA

目的 :研究CpG免疫刺激DNA中免疫刺激序列 (ISS)的数量与免疫刺激效果间的关系。方法 :将 3种CpGODN(分别含 2 ,4,8个ISS)与甲型肝炎灭活疫苗混合 ,通过腹腔注射途径免疫小鼠 ,于免疫后 4,6 ,8w采血 ,用全自动微粒子酶联免疫测定分析检测抗HAVIgG水平。结果 :含 2个ISS的CpGODN具有较好的佐剂效应 ,能增强甲型肝炎灭活疫苗的免疫效果 ,含 4个ISS的CpGODN不具有免疫刺激作用 ,而含 8个ISS的CpGODN则抑制了甲型肝炎灭活疫苗所诱导的免疫反应。结论 :适当长度的CpGODN(含 2个ISS)可作为有效的佐剂来增强疫苗的免疫效果 ,而当其中的ISS含量过多 。

Objective:To investigate the relationship between the immunostimulatory effects and the number of immunostimulatory sequence(ISS) in CpG immunostimulatory DNA.Methods:Mice were immunized by HAV inactive vaccine together with three kinds of CpG ODN which contains 2,4,8 ISS respectively by intraperitoneal injection. At the weeks 4,6,8 after immunization, peripheral blood was obtained from the tail vein of mice and assayed for anti HAV IgG by the fully automated microparticle enzymeimmunoassay.Results:The immunogenicity of HAV inactive vaccine can be boost up by CpG ODN which contains 2 ISS, CpG ODN which contains 4 ISS did not show any immunostimulatory effect, act as an adjuvant, but CpG ODN which contains 8 ISS showed inhibiting the immunological effect of the vaccine.Conclusion:Adequate length of CpG ODN(when it contains 2 ISS) can act as an effective immunostimulator adjuvant, and it might decrease the immunogenicity of the vaccine if it contains much more ISS.