端粒酶催化亚基 h-TRT 在胆囊良、恶性病变中的表达及意义

Expression of h-TRT gene in benign and malignant gallbladder lesions and its significance

目的探讨端粒酶的催化亚基 h-TRT(human telomerase reverse transcriptase)在胆囊腺瘤恶变及不同病理分型、临床病理分期的胆囊癌以及胆囊良性病变中的表达及其意义。方法用原位杂交法检测 h-TRT 在胆囊良、恶性病变中的表达及特点。结果 h-TRT 在胆囊恶性病变组中总的阳性率为81.3%,在不同组织分型中阳性率为50%～100%,在不同病理分期中阳性率为66.7%～100%;而在良性病变中无表达。h-TRT 在腺瘤恶变,低分化以及晚期胆囊癌中表达较强。结论 h-TRT 的表达与胆囊恶性病变密切相关,其表达与否与性别、年龄、组织分型和病理分期无关。h-TRT 的表达增强与腺瘤恶变,低分化以及晚期胆囊癌有关。h-TRT 可能对胆囊癌的诊断及治疗有一定指导意义。

Objective To investigate expression of h-TRT gene in gallbladder adenoma carcino- genesis,gallbladder carcinoma of different patho-histological classification or Nevin stages as well as benign lesions of the gallbladder and its significance.Methods The expression of h-TRT gene in ma- lignant and benign lesions of the gallbladder was detected by in situ hybridization.Results The posi- tive rate of h-TRT in malignant gallbladder lesions was 81.3%.It was 50%～100% in gallbladder carcinoma of different histological patterns and 66.7%～100% in that of different Nevin stages.How- ever,the expression was not detected in benign lesions.The signals in adenoma carcinogenesis,poorly differentiated and Nevin stage-Ⅳ and stage-Ⅴ gallbladder carcinoma were stronger.Conclusions The expression of h-TRT is strongly correlated to malignant gallbladder lesions but not to age and sex of patients as well as patterns and stages of the tumor.The intensity of h-TRT expression is correlated to carcinogenesis.Therefore,the detection of h-TRT expression is helpful for diagnosis and treatment of gallbladder carcinoma.