米非司酮和孕酮对子宫内膜异位症患者子宫内膜白细胞介素6分泌的影响

Regulatory effects of mifepristone and progesterone on the secretion of interleukin-6 by cultured eutopic and ectopic endometrial cells

目的 探讨米非司酮和孕酮对子宫内膜异位症 (内异症 )患者异位子宫内膜 (异位内膜 )与正常子宫内膜 (在位内膜 )白细胞介素 6 (IL 6 )分泌的影响。方法 采用酶联免疫吸附试验 (ELISA)方法 ,检测米非司酮浓度为 1× 10 -6mol/L(米非司酮 1组 )、1× 10 -4 mol/L(米非司酮 2组 ) ,和孕酮浓度为 1× 10 -7mol/L(孕酮 1组 )、1× 10 -5mol/L(孕酮 2组 )与体外培养的异位内膜细胞和在位内膜细胞上清液作用后的IL 6水平。结果 米非司酮可降低异位内膜细胞IL 6水平 ,米非司酮 1组和米非司酮 2组的IL 6分为 (1914 33± 799 2 8) μg/L和(990 2 5± 5 8 40 ) μg/L ,两组与空白组比较 (下同 ) ,差异有极显著性 (P <0 0 1)。孕酮也可降低异位内膜细胞IL 6水平 ,孕酮 1组和孕酮 2组的IL 6分为(2 5 75 89± 119 75 ) μg/L和 (1736 2 5± 75 0 89) μg/L(P <0 0 1)。米非司酮还可使在位内膜细胞IL 6水平降低 ,其中米非司酮 1组和米非司酮 2组分别为 (34 6 96± 2 4 32 ) μg/L和 (2 70 2 2± 36 15 ) μg/L(P <0 0 1)。孕酮对在位内膜细胞IL 6水平无明显影响 (P >0 0 5 )。结论 米非司酮和孕酮可抑制异位内膜和在位内膜细胞IL

Objective To investigate the regulatory effects of mifepristone and progesterone on the secretion of interleukin 6 (IL 6) by endometrial and endometriosis cells in vitro. Methods Primary cultures of eutopic and ectopic endometrial cells from 9 cases of endometriosis were exposed to mifepristone (10 -6 mol/L, 10 -4 mol/L) and progesterone (1×10 -7 mol/L, 1×10 -5 mol/L) respectively. IL 6 secretion was analyzed in the culture medium by enzyme linked immunosorbent assay (ELISA). Results Mifepristone inhibited the IL 6 secretion of ectopic endometrial cells, with the concentrations of IL 6 was (1 914 33±799 28) μg/L in the 1×10 -6 mol/L group ( P <0 01) and (990 25±58 40) μg/L in the 10 -4 mol/L group ( P <0 01). Progesterone also inhibited IL 6 secretion of ectopic endometrial cells, with (2 575 89±119 75) μg/L in the 1×10 -7 mol/L group ( P <0 05) and (1 736 25±750 89) μg/L in the 10 -5 mol/L group ( P <0 01). Mifepristone restrained the secretion of IL 6 by eutopic endometrial cells, with (346 96±24 32) μg/L in the 1×10 -6 mol/L group ( P <0 01) and (270 22±36 15) μg/L in the 1×10 -4 mol/L group ( P <0 01). All the effects of down regulation were more obvious when the concentrations of mifepristone and progesterone increased ( P <0 05～0 01). Secretion of IL 6 by eutopic endometrial cells did not change significantly when incubated with progesterone ( P >0 05). Conclusion The inhibitory effects on the secretion of IL 6 by ectopic and (or) eutopic endometrium may provide one of the cellular therapeutic mechanisms of mifepristone and progesterone on endometriosis.