利多卡因和丙泊酚抗红细胞过氧化损伤作用的比较

A comparison of protective effect of lidocaine with propofol on the peroxidative injured erythrocyte

目的 比较利多卡因和丙泊酚对过氧化氢 (H2 O2 )损伤红细胞 (RBC)的保护作用。方法 采 2 0例健康人静脉血制成 2 5 %RBC悬液 ,均分为空白对照组、H2 O2 损伤组及损伤加不同浓度(0 0 1、0 0 2及 0 0 3g/L)利多卡因组和损伤加不同浓度 (2 5、5 0及 75 μmol/L)丙泊酚组。孵育后测定RBC悬液中K+ 浓度、丙二醛 (MDA)浓度和RBC溶血度。结果 孵育 6 0分钟 ,利多卡因三组K+ 浓度 (0 39,0 44及 0 35ppm)、MDA浓度 (7 0 9,6 49及 5 98nmol/L)和RBC溶血度 (87 0 4% ,83 49%和 87 5 1% )及丙泊酚三组K+ 浓度 (0 30 ,0 2 8和 0 2 7ppm)、MDA浓度 (5 6 6 ,5 5 7和 6 2 0nmol/L)和RBC溶血度 (76 89% ,5 9 84%和 6 4 2 2 % )与单纯损伤组 (分别为 0 5 1ppm ,9 19nmol/L和10 0 0 0 % )相比均显著降低 ,且丙泊酚组比利多卡因组下降显著 ;孵育 90分钟 ,利多卡因三组K+ 浓度 (1 6 6 ,1 6 4和 1 5 3ppm)及丙泊酚三组K+ 浓度 (0 71,0 6 5和 0 6 2 ppm)与单纯损伤组 (2 2 0 ppm)相比均显著降低 ,丙泊酚组比利多卡因组下降更显著。结论 临床相关浓度的丙泊酚溶液对H2

Objective To compare the protective effect of lidocaine with propofol on the peroxidative injured red blood cell(RBC).Methods Intravenous blood samples taken from 20 healthy adults were prepared for 25% RBC suspensions and divided equally into 8 groups:group Ⅰ for control,group Ⅱ with H 2O 2 induced injury and group Ⅲ,Ⅳ,Ⅴ with the same injury as the group Ⅱ after being pretreated with 3 different concentrations of lidocaine (0 01,0 02,0 03mg/ml) respectively and group Ⅵ,Ⅶ,Ⅷ with the same injury as the group Ⅱ after being pretreated with 3 different concentrations of propofol (4 45,8 9,13 35μg/ml)respectively.The concentrations of potassium and malondialdehyde (MDA) in RBC suspensions and hemolytic degree after incubation were measured respectively.Result After 60min incubation,the extracellular potassium concentrations (0 39,0 44,0 35ppm),MDA concentrations(7 09,6 49,5 98nmol/L) and hemolytic degree (87 04%,83 49%,87 51%)in lidocaine groups and the extracellular potassium concentrations (0 30,0 28,0 27ppm),MDA concentrations(5 66,5 57,6 20nmol/L)and hemolytic degree (76 89%,59 84%,64 22%)in propofol groups were decreased significantly as compared with the group Ⅱ (0 51 ppm,9 19nmol/L and 100 00%),which was more significantly in propofol groups than that in lidocaine groups.After 90 min incubation,the extracellular potassium concentrations (1 66,1 64,1 53ppm)in lidocaine groups and (0 71,0 65,0 62ppm) in propofol groups were decreased significantly as compared with the group Ⅱ (2 20ppm),which was more significantly in propofol groups than that in lidocaine groups.Conclusion The protective effect of propofol on the peroxidative injured erythrocyte is stronger than that of lidocaine.