p185、ras p21、p53、p21、Rb蛋白在子宫内膜癌组织中的表达及其意义

Expression and Clinical Significance of p185,rasp21,p53,p21,Rb Proteins in Endometrial Carcinoma

目的 研究与细胞周期G1→S调控点相关的p185、rasp2 1、p5 3、p2 1、Rb蛋白在子宫内膜癌组织中的表达及相互间的关系 ,并探讨其临床应用价值。方法 应用LSAB免疫组化法 ,检测 2 1例正常子宫内膜、19例子宫内膜上皮内瘤样病变 (EIN )及45例子宫内膜癌组织中 p185、rasp2 1、p5 3、p2 1、Rb蛋白的表达。 结果 p185、rasp2 1、p5 3蛋白在正常子宫内膜、EIN至内膜癌组织中的表达率依次增高 ,p2 1、Rb蛋白表达率则依次降低 ;在子宫内膜癌组织中 ,p185蛋白表达与rasp2 1蛋白表达呈正相关 (γ =0 .36 3) ,与 p5 3、p2 1表达呈负相关 (γ =-0 .448;γ =-0 .30 3) ,rasp2 1,p5 3蛋白表达与p2 1蛋白表达均呈负相关 (γ =-0 .36 5 ;γ= -0 .32 2 ) ,p2 1蛋白与Rb蛋白表达呈正相关 (γ =0 .441)。p185蛋白的表达与子宫内膜癌病理分级有关 ,单因素分析其为 1个预后保护性因素 (P <0 .0 5 ) ;p5 3蛋白的表达与各临床病理因素均有关 ,其单因素及多因素分析均提示预后差 (P <0 .0 5 )。 结论 与细胞周期G1→S调控相关的 p185、rasp2 1、p5 3、p2 1、Rb蛋白均参与子宫内膜癌的发生、发展过程 ,且部分基因相互关联 ;p5 3可作为其独立的预后因素。

Objective To study the expression and interrelationship of the p185,rasp21,p53,p21,Rb regulatory proteins related to G_1→S period of cell cycle in endometrial carcinoma and to investigate their clinical significance.Methods The expressions of those proteins were investigated by immunohistochemical method in 21 normal endometrial tissuses ?19 EIN and 45 edometrial carcinoma tissuses.Results The positive expression rates of p185,ras p21 and p53 increased gradually from normal group and EIN group to cancer group,while it was inverse for p21 and Rb.In endometrial carcinoma group,expressions of p185 and p53,p21 were negatively correlated(γ=-0.448;γ=-0.303),expressions of p185 and ras p21 were positively correlated(γ=0.363),expressions of ras p21,p53 and p21 were negatively correlated(γ=-0.365;γ=-0.322),expressions of p21 and Rb were positively corre lated(γ= 0.441).expression of p185 showed significant correlation with the histological type and better prognosis of endometrial carcinoma based on single analys(P<0.05);expression of p53 was associated with clinicopathological charactristics of the patients,and implicated a poor prognosis as an independent predictor of survival based on single and multivariate analysis(P<0.05).Conclusion The p185,ras p21,p53,p21,Rb regulatory protein related to G_1→S period of cell cycle may play some roles in the carcinogenesis and development of endometrial carcinoma,and Some of them act with each other;the expression of P53 as an independent prognostic factor should be used in patients with endometrial carcinoma.