中山地区应用体外受精技术治疗不育症的初步报告

Preliminary report on in vitro fertilization program in Zhongshan City

目的 :初步报告中山市应用体外受精技术 (IVF)治疗不育症的效果。方法 :2 8对不育夫妇的男方在精液常规检查的基础上 ,进行精子形态学和顶体状态分析。 2 8例受术妇女采用Buserelin/FSH/HCG黄体期长方案控制超排卵 ,并根据不孕病因、患者年龄及卵巢基础FSH/LH比值指导控制性超排卵用药 ,比较周期取卵率、受精率、卵裂率及临床妊娠率。结果 :基础FSH/LH比值正常反应组问收卵率高于低反应组 (P <0 .0 5 )。取卵 2 91个 ,平均每周期取卵 9.6个 ;受精率 81.8% ;卵裂率 79.8%。 9例临床妊娠 ,5例生化妊娠 ,妊娠率 32 %。结论 :IVF技术是治疗不育症的有效手段 ,本文只做丈夫精子IVF ,其正常精子形态率和顶体完整率对预测受精有指导意义 ,根据基础FSH/LH比值判断卵巢储备功能及指导超排卵用药 ,可避免过大。

Objective:To evaluate the preliminary application of in vitro fertilization (IVF) on treating infertile couples in Zhong shan City.Methods:28 couples were involved in this study.Based on semen routine test,sperm samples were examined by sperm morphology analysis and soerm acrosome assay.Patients underwent controlled ovarian stimulation with GnRH analogue and pure FSH with the use of conventional down-regulation protocols.Results:The rate of oocytes recovered in the normal ovarian response(FSH/LH<2) group was higher than that of the poor ovarian response (FSH/LH>4) group (P<0.05).The total number of oocytes recovered was 291 oocytes.The fertilization rate and the cleavage rate were 81.8%,and 79.8%,respectively.9 women obtain in ongoing pregnancies,and 5 women have biochemical pregnancies.The rate of pregnancy was 32%.Conclusions:IVF is effective for treating infertile couples.Sperm normal morphologh rate and acrosome integrity rate could predict fertilization rate.Criteria of FSH/LH would indicate an individualized dose response protocol,which could help to reduce the risk of ovarian hyperstimulation or poor response.