肝癓口服液含药血清对转化生长因子α诱导人肝癌细胞SMMC-7721增殖和凋亡的研究

Serapharmacological Study of Ganzheng Peroral Solution on Proliferation and Apoptosis of Human Hepatocellular Carcinoma SMMC-7721 Cells with Transforming Growth Factor α

目的 :观察肝疒徵 口服液含药血清对转化生长因子 (TGFα)诱导人肝癌细胞增殖和凋亡的影响。方法 :采用血清药理学方法 ,以病理鼠血清作对照 ,应用MTT比色法观察中药鼠血清对TGFα诱导人肝癌细胞SMMC 772 1增殖的抑制作用。用流式细胞术检测中药血清对肝癌细胞凋亡和细胞周期的影响。结果 :中药血清对TGFα诱导的肝癌细胞SMMC 772 1增殖有明显的抑制作用 ,抑制效应呈时间依赖性。中药血清能促进肝癌细胞凋亡 ,使细胞滞留于G0 1 期 ,降低S期细胞百分比和增殖指数。结论 :肝疒徵 口服液含药血清能够抑制TGFα诱导的人肝癌细胞增殖 ,促进其凋亡。

Objective: To investigate the serapharmacological effects of Ganzheng Peroral Solution(GZPS) on proliferation and apoptosis of human hepatocellular carcinoma SMMC 7721 cells with transforming growth factor α(TGFα).Methods:The effects of GZPS were investigated in vitro using serum pharmacological approach compared with pathologic serum. The inhibiting effect of drug serum on SMMC 7721 cells with TGFα was observed by MTT assay. Cell apoptosis and cell cycling analysis were detected by flow cytometry.Results: The drug serum could significantly inhibit the proliferation of SMMC 7721 cells with TGFα time dependently. It also could increase the apoptosis rate and arrest cell cycle at G 0/1 phase so that it could decrease S phase fraction(SPF) and proliferous index(PI).Conclusion:The serum containing GZPS could inhibit the proliferation of SMMC 7721 cells with TGFα and increase the apoptosis rate.