健中愈疡片对半胱胺性溃疡大鼠胃壁细胞凋亡影响的实验研究

Experimental Study on the Influence of Jianzhongyuyang Pill on Apoptosis of Gastric Parietal Cell in the Cysteine-induced Chronic Duodenal Ulcer

目的 :观察健中愈疡片对半胱胺性溃疡胃壁细胞凋亡的影响。方法 :采用皮下注射半胱胺酸法制造慢性十二指肠溃疡模型 ,取 6 0只SD大鼠随机分成雷尼替丁组、健中高组、健中中组、健中低组、模型组、空白组 6组 ,以原位末端标记技术研究胃壁细胞在溃疡愈合阶段的凋亡情况。结果 :十二指肠溃疡发生后 2 8天 ,胃壁细胞的凋亡呈现凋亡活跃的情况。其凋亡指数大大高于正常组及健中中和健中高组 (P <0 .0 1) ,而雷尼替丁组经治疗后其凋亡指数也仍保持在造模组的高凋亡水平。中药治疗组呈现出量效相关的调节效应 ,使胃壁细胞的凋亡状态接受或回复正常水平。结论

Objective:To study the influence of Jianzhongyuyang pill on apoptosis of gastric parietal cell in the cysteine induced chronic duodenal ulcer. Methods: L Cysteine hydrochloride subcutaneous injection was applied to induce chronic duodenal ulcer on 60 SD rats which have been randomly divided into 6 groups in advance, namely Ranitidine,low dosage Jianzhong, medium-dosage Jiangzhong, high dose Jianzhong,model and normal group. TUNEL was carried out to detect the apoptosis of gastric parietal cells under the ulcerous condition. Results: 28 days after the induction, active apoptosis which appeared in gastric parietal cells of the model group was significantly higher than that of the normal, medium dosage Jianzhong and high dosage Jianzhong groups (P<0.01).While the apoptosis level of Ranitidine group remained the same as that of the model group after treatment (P> 0.05 ). The groups treated with displayed positive dosage dependant regulatory effect.And the Chinese medicine could restore the pathologically active apoptosis state of gastric parietal cells to a normal level. Conclusion: Jianzhongyuyang pill could have good regulartory effect to the apoptosis of gastric parietal cells under high acid secretion state.