厌食患儿外周血淋巴细胞凋亡过程及临床意义

Anorexia Children the Course of Lymphocyte-apoptosis in Peripheral Blood and Clinical Significance

目的 :检测厌食患儿免疫功能 ,探讨外周血淋巴细胞凋亡 (PCD)过程。方法 :检测 5 8例厌食患儿外周血凋亡淋巴细胞和免疫功能 ,并与 2 8例健康儿童对照。T淋巴细胞亚群采用间接免疫荧光法 ,免疫球蛋白采用免疫比浊法 ,细胞凋亡用口丫 啶橙 溴化乙锭染色法。结果 :该组患者免疫功能低下 ,主要表现为IgG、CD+4、CD+4/CD+8明显降低 ,IgM、CD+8明显升高。PCD率明显升高。结论 :PCD过度是该组患者免疫功能低下的一个重要原因。

Objective:To detect the immune funtion of anorexia children and discuss the course of the lymphocyte apoptosis in peripheral blood.Methods:T lymphocyte was detected with indirect immunofluorescence,immunoglobulin with immune turbidimetry,cell apoptosis with ethidium bromide tinction.Results:The immune function of these patients decrease,the apoptosis rate of peripheral blood lymphocyte appeartly increase.Conclusion:One of the important reasons to the decreased immune funtion is the excessive cell apoptosis of the lymphocyte in peripheral blood.