过继免疫疗法(AIT)与羟基喜树碱(HCPT)序贯应用治疗膀胱癌的实验研究

Experimental study on the effect of sequential combination of AIT and chemotherapy(HCPT) for murine bladder cancer

目的 了解过继免疫疗法 (AIT)与羟基喜树碱 (HCPT )序贯应用对膀胱肿瘤的作用。方法 体外实验测定TIL及TIL与HCPT联合应用对不同靶细胞的杀伤活性 ,体内实验了解不同治疗方法对小鼠膀胱肿瘤的治疗效果。结果 TIL对同种异体膀胱肿瘤细胞有强大的杀伤活性 ,对正常组织细胞无毒性 ,TIL与HCPT联合应用对肿瘤细胞的杀伤毒性低于单独应用时的杀伤活性。体内实验说明同时应用TIL及HCPT无明显疗效 ,而合理联合应用即序贯疗法有强大的抗癌作用。结论 通过本实验说明过继免疫疗法与HCPT序贯应用才能起到抗癌作用 ,其可能机理是序贯疗法充分发挥HCPT化疗作用又不影响TIL的杀伤活性 ,并且有促进TIL聚积于肿瘤周围的效果。

Objective To explore the feasibility of treating bladder cancer with AIT and chemotherapy by using TIL cell plus HCPT in vitro and in vivo.Methods The cytotoxicity activity to the different target cell by using TIL and TIL plus HCPT in vitro was determined,and the effects of the different treating methods on mice bladder cancer were studied in vivo.Results The experiment showed that the TIL cell exhibited the higher specific cytotoxicity to mice bladder cancer cells,but no cytotoxicity to normal cells.TIL concurrently combined with HCPT showed no obvious anti tumor activity.But sequential combination of AIT and HCPT showed significant anti tumor effects.Conclusion We conclude that the rational use of TIL in combination with chemotherapy has significant anti tumor effects.