脑梗死患者血浆纤溶酶原激活物抑制物-1活性及其基因多态性研究

The study of plasma PAI-1 activity and it's gene polymorphism in cerebral infarction

目的 :探讨纤溶酶原激活物抑制物 - 1(PAI- 1)活性变化和其基因多态性对脑梗死及其再梗死发病的关系。方法 :采用扩增片段长度多态性及银染技术 ,分析 95例脑梗死患者和 6 0例对照者的白细胞 PAI- 1启动子区域 - 6 75位点 4G/5 G多态性和第四内含子 (CA) n多态性 ,用发色底物测定血浆 PAI- 1活性。结果 :脑梗死组血浆 PAI- 1活性明显高于对照组。再梗死组和初次脑梗死组中 4G纯合子个体占的比例最高 ;并且再梗死组 4G纯合子个体的比例高于初次脑梗死组 (P<0 .0 5 )。脑梗死组和对照组相比在“Z”,“Z+6”,“Z+8”,“Z+10”等位基因频率存在差异 (P<0 .0 5 ) ;在脑梗死组中 ,较短片段的 (CA) n重复和较重复基因型及

Abstract: To investigate the changes of plasma PAI 1 activity and its gene polymorphism in cerebral infarction. Methods: Using amplified fragment length polymorphism assay and silver staining measured in 95 patients with cerebral infarction (CI) and 60 controls. The 4G/5G polymorphism at position 675 of promoter and CA dinucleotide repeat polymorphism in the fourth intron were genetyped by PCR. PAI lactivity was assayed by ELISA. Results: The plasma activity of PAI 1 in patients with CI was significantly higher than those of controls. In patients group, the percentage of 4G homozygotes was the highest and there was difference between cerebral infarction group and recurrence of cerebral infarction (RCI). As to CA dinucleotide repeat polymorphism in the fourth intron, there was significant difference of alleles frequencies such as'Z', 'Z+6', 'Z+8'and 'Z+10'between the groups of CI and controls. Otherwise, the shorter alleles associated with higher PAI 1activity. Conclusion: PAI 1 activity abnormal high was one of the risk factors for CI. Persons with shorter CA repeat alleles and 4G homozygotes maybe have higher incline of CI and RCI.