局灶性脑缺血/再灌注大鼠白细胞介素-1β蛋白和mRNA的表达

The Expression of Interluki-β protein and mRna after Cerebral Ishcemia in Rats

目的 :观察脑缺血对白细胞介素 - 1β(IL- 1β)表达的影响 ,及脑缺血后 IL- 1β的细胞来源。方法 :采用线栓法制备大鼠局灶性大脑中动脉栓塞 (MCAO)模型 ,应用原位杂交观察脑缺血再灌注对 IL- 1β m RNA表达的影响 ;应用荧光双标检测 IL- 1β的表达细胞。结果 :(1)正常和假手术大鼠大脑皮层 IL- 1β m RNA阳性细胞表达较少 ,缺血再灌注后缺血侧皮层 IL- 1β m RNA阳性细胞表达明显增加 ,再灌注后 2 h IL- 1β m RNA表达显著增加 ,再灌注后 2 4h逐渐降至正常水平。 (2 )缺血后表达 IL- 1β m RNA的主要细胞为神经元、星形胶质细胞和小胶质细胞。缺血再灌注后12 h IL- 1β蛋白主要表达于星形胶质细胞和小胶质细胞 ,神经元未见 IL- 1β的表达。结论 :脑缺血后 IL- 1β m RNA表达增加 ,IL- 1β可能在缺血性脑损伤中起重要作用。缺血再灌注后 IL-

Abstract:To investigate the expression of Interleukin β (IL β) mRNA in cerebral ischemia rat, and identify the cellular source of IL 1β. Methods: Using in stiu hybridization technique to explore the expression of IL 1β mRNA in ischemic cortex of rats. To identify cellular source of IL 1β mRNA and protein, hybridization and double immunofluorescence labeling were used. Results: ①The normal and sham rats showed a weak expression of IL 1β mRNA in cortex (70.3±7.3,80.4±5.5).The positive cells of IL 1β mRNA were increased significantly after 2h, 6h, 12h reperfusion (120.2±13.5; 112.6±8.8; 106.6±9.0), and sustained to 24 h after reperfusion (84.6±7.8). ②Most of neurons expressed IL 1β mRNA, IL 1β mRNA also expressd in astrocytes and microglia. IL 1β protein coexpressed with astrocytes and microglia. We didn't detect the coexpression of IL 1β protein and neurons. Conclusion: It indicated that the expression of IL 1β mRNA was increased after cerebral ischemia, IL 1β may play an important role in pathogenesis of ischemic injury. The main source of IL 1β may be astrocytes and microglia.\;