乌司他丁对鼠应激性胃溃疡的保护作用

PROTECTIVE EFFECT OF ULINASTATIN ON STRESS-INDUCED GASTRIC ULCERATION IN RATS

目的探讨乌司他丁对应激性胃粘膜损伤的保护作用及其机制。方法雄性Wistar大鼠 2 0只 ,随机分为对照组与乌司他丁预处理组。以冷冻束缚法建立应激性溃疡模型 ,分别测定血浆和胃粘膜组织丙二醛及血浆肿瘤坏死因子 α(TNF α)含量 ,并行胃粘膜病理检查。结果乌司他丁预处理可显著减少胃粘膜溃疡指数 (与对照组比较 ,P <0 .0 5 ) ,降低血浆和胃粘膜组织中丙二醛含量及血浆TNF α水平 (P<0 .0 5和 P <0 .0 1)。结论乌司他丁对冷冻束缚引起的应激性胃粘膜损伤有保护作用 ,其机制可能是通过清除自由基和抑制炎性细胞因子来实现的。

ObjectiveTo investigate the protective effects of ulinastatin (UTI) on stress induced gastric mucosal lesions and it's possible mechanism.MethodsMale Wistar rats were randomly assigned to control group and UTI treated group. The stress ulceration was produced by cold restraint. The malondialdehyde (MDA) level in gastric mucosal tissue and plasma ,and the tumor necrosis fator α(TNF α) level in plasma were measured. Gastric mucosal lesions were examined.ResultsPretreatment with UTI alleviated the stress induced gastric mucosal ulceration index (P< 0.05 vs control group),decreased levels of MDA in the gastric mucosal tissuc and plasma, and TNF-α in plasma (P< 0.05 , P< 0.05 and P 0.01 ).ConclusionUTI has protective effect on gastric mucosal lesions by cold restraint stress in rats. The potential mechanism may be associated with reduction of free radicals and alleviation of pro inflammatory cytokines.