IFN-γ对人肝癌细胞侵袭及转移的调节作用

Modulation on the invasion and metastasis of human hepatocellular carcinoma by IFNγ in vitro

用IFN γ处理肝癌细胞 ,采用流式细胞仪分析肝癌细胞表面抗原分子的表达水平 ,用附壁试验检测肝癌细胞对细胞外基质的亲和力 ,用细胞聚集试验检测肝癌细胞的聚集能力 ,以研究IFN γ对肝癌细胞侵袭力及转移力相关因素的调节作用。结果表明 :经高浓度和低浓度IFN γ处理后 ,肝癌细胞表面ICAM 1及HLA I表达均增加 ,CD44表达减少 ,肝癌细胞对细胞外基质的亲和力及肝癌细胞聚集程度均降低。提示IFN γ可明显增加肝癌细胞ICAM 1及HLA I的表达 ,抑制CD44表达 ,抑制肝癌细胞对细胞外基质的亲和力及肝癌细胞的聚集作用。因此可抑制肝癌细胞的侵袭力和转移力。

To investigate the effects of Interferon gamma(IFN γ)on the in vitro invasiveness and the expression of several cell surface antigens related to invasive and metastatic potentials of human hepatocellular carcinoma QGY 7701 cell line The expression of ICAM 1, CD44 and HLA I was examined by fluorescence activated cell sorter (FACS), and tumor cell binding affinity to extracellular matrix (ECM) components was measured by cell attachment assay, the degree of homotypic aggregation was quantified by cell aggregation assay The results showed that IFN γpretreatment exhibited enhanced expression of ICAM 1 and HLA I, suppression of CD44 on hepatocellular carcinoma cell line and decreased binding affinity to ECM components and the degree of homotypic aggregation of hepatocellular carcinoma cells It is suggested that IFN γ inhibits the invasive and metastatic potentials of hepatocellular carcinoma cells