摘要
研究一氧化氮 (NO)在哮喘大鼠肺组织中的作用。采用组化法观察一氧化氮合酶 (NOS)在大鼠哮喘模型肺组织中的分布 ,应用免疫组化法观察大鼠哮喘模型气道mIL 2R+ 细胞变化。结果显示 ,哮喘大鼠肺NADPH染色呈强阳性 ,并波及肺泡膈。肺组织中NOS含量明显高于对照组 [哮喘组 (37 44± 0 77)pmol/mg,对照组 (8 73± 0 79)pmol/mg],气道炎性细胞增多 ,特别是mIL 2R+ 细胞 [哮喘组mIL 2R+ 细胞为 (2 3 8± 7 9)个 ,对照组为 0个 ],而NOS抑制剂DMA组气道炎性细胞少 ,NADPH呈阴性。提示NO是哮喘大鼠的炎性效应分子。
To investigate the role of nireic oxide (NO) in bronchial asthma, histochemical and immunohistochemical methods were used to find the distribution of nitric oxide synthase (NOS) in the rat asthma model and the changes of membrane interleukin 2 receptor positive lymphocytes. NADPH was found to be a histochemical marker which reflecting NOS activity. Meanwhile there was an obvious increase of NOS activity in asthmatic rat as well as in alveolar septum. The NOS level of asthmatic rat was far more than that of control [asthmatic group(37.44±0.77)pmol/mg,control group(8.73±0.79) pmol/mg]. Airway inflammatory cell numbers of asthmatic rats were increased and mIL 2R positive lymphocytes were significantly increased (asthmatic group 23.8±7.9 vs control group nil ), but lower in the DMA group. NO was the inflammatory effector molecule of asthmatic rat.
出处
《基础医学与临床》
CSCD
北大核心
2001年第6期547-550,共4页
Basic and Clinical Medicine
