人羊膜基底膜侵袭模型观察人舌鳞癌体外侵袭行为

Invasion of Human Squamous Cell Carcinoma of the Tongue to Human Aminion basement Membrane

目的 :观察舌鳞癌细胞侵袭基底膜的过程及形态学特征。方法 :细胞培养装置充满培养液 ,并被预制的人羊膜基底膜分为上、下两室 ,将人舌鳞癌细胞置于上室。经 4 8～ 72h后 ,利用免疫组化染色、冷冻断裂技术 ,在光镜和电镜下观察癌细胞的改变及对基底膜的侵袭行为。结果 :生长在基底膜上的癌细胞表面丝状伪足明显增多 ,癌细胞周围有基底膜的溶解破坏 ,癌细胞显示活跃的侵袭能力 ,侵袭进入或穿过基底膜。结论 :人舌鳞癌侵袭人羊膜基底膜时 ,与体内侵袭行为较一致 。

Objective: Our aim was to observe the characteristic of invasion of human squamous cell carcinoma to human aminion basement membrane barrier. Methods: In vitro stromal matrix assay of human aminion basement membrane was constructed. The assay was divided into upper and lower chambers. The whole assay was fulfilled with RPMI 1640 culture fluid. The carcinoma line incubated in upper chamber for 48 to 72 hours. Results: The invasive cells exhibited longer filopodia than the parental cells, and penetrated into or through the basement membrane(BM) stromal matrix. Scanning electron microscope showed some breaching and dissolution of the BM at the site of tumor invasion .The BM was discontinuous at the invasion front, which showed by type IV collangen immunohistochemistric stainning. Conclusion: Human aminion basement membrane stromal matrix assay was a useful model for observation of characteristic of invasion of cancer cell line in vitro, and similar with the invasive behaviour in vivo.