摘要
目的评价琼脂培养MTT法 (简称MTT法 )测定肿瘤细胞对化疗药物敏感性的临床应用价值。方法应用人肿瘤干细胞集落形成能力测定 (HTSCA)和MTT法同时对人肺癌细胞进行药物敏感性测定 ,并对结果进行共同相关性分析 ;同时 ,回顾性观察MTT法测定卵巢癌患者对顺氯氨铂 (DDP)的体外敏感性与临床疗效关系 ;前瞻性观察通过MTT法测定药物敏感性结果指导临床选择有效药物对肺癌化疗效果的影响。结果①MTT比色法和HTSCA同时可供药敏分析的药物浓度次是 12 6 ,两法测定结果的总符合率是 92 .8% ( 117/12 6 )。②MTT法测定的卵巢癌细胞对DDP体外敏感性与临床疗效相关 ,总符合率是 92 % ( 2 4/2 6 )。③MTT法药敏预测指导人肺癌化疗组临床有效率 ( 91.2 % ) ,较常规方案化疗组 ( 5 3.3% )明显增高 (P〈0 .0 5 )。结论MTT法测定人实体瘤体外药物敏感性具有指导临床化疗的价值。
ObjectiveTo evaluate the clinical application of chemosensitivity testing to fresh human tumor cells by MTT assay in agar culture. MethodsThe human tumor stem cell colony assay (HTSCA) and MTT assay in agar culture were used simultaneously for in vitro drug sensitivity testing of fresh lung cancer specimens, the correlation between the assayed results was analyzed. Relationship between in vitro chemosensitivity of ovarian cancer cells to cisplatin (DDP) measured by MTT assay in agar culture and clinical efficacy of patients with ovarian cancer was observed retrospectively. Effects of chemosensitivity testing (MTT assay) results for guidance of clinical choice in efficient chemotherapeutic agents on the clinical responses of patients with lung cancer were observed prospectively.Results① The total concordance rate of results in MMT assay with those of HTSCA was 92.8%(117/126).②18 out of 26 ovarian cancer specimens were sensitive to DDP in vitro, 16 cases had clinical remission, and 8 cases resistant to DDP showed no clinical response. The total concordance rate was 92%(24/26). ③The clinical remission (CR+PR) rate of patients with lung cancer was 91.2%(31/34) when the chemotherapeutic scheme was guided by drug sensitivity testing of MTT assay, much higher than that of patients treated by routine chemotherapeutic scheme [53.3%(16/30)](P<0.05).ConclusionThe in vitro chemosensitivity testing of fresh human tumor specimens by MTT assay in agar culture shows valuable significance in guiding clinical chemotherapy. [
出处
《湖南医学》
2001年第6期414-416,共3页
Hunan Medical Journal
