肝泡型包虫病临床分期与sIL-2R、TNF-α和IFN-γ含量的关系

The Relationship Among Pathology of Alveolar Echinoccocosis and Level of sIL-2R, TNF-α and IFN-γ in Sera

为探讨肝泡型包虫病 (Alveolar echinococcosis AE)临床分期与 s IL- 2 R,TNF- α和 IFN- γ含量的关系和意义 ,将经B超和血清学方法证实的 2 3例 AE病人和对照组 1 2人采血后 ,用 ELISA双抗体夹心法 ,做 s IL- 2 R,TNF-α和 IFN-γ含量的测定。按 B超肝脏扫描的声像损害情况 ,将 AE分期 (组 ) ,对照相应细胞因子的含量 ,经方差分析和 Q检验 ,讨论细胞因子在 AE发育增殖中的可能作用和临床意义。结果 P2 组、P3 组、P4组和对照组 s IL- 2 R每 m L含量均值分别为 96.57u、2 2 6.44u、1 93.81 u、1 1 0 .89u;TNF- α含量均值分别为 P2 组 1 .1 2 μg/ L,P3 组 3.67μg/ L,P4组 1 .30 μg/ L,对照组则为 0 .40μg/ L;IFN-γ含量均值分别为 P2 组 360 ng/ L,P3 组 486 ng/ L,P4组 2 59ng/ L,对照组则为 1 5.63 ng/ L;各组经方差分析和 Q检验 ,证实 AE病人病理的三个不同时期 ,其 s IL- 2 R,TNF- α和 IFN- γ的含量均有显著性差异 (P<0 .0 1 )。 s IL- 2 R含量均值除 P2 与对照组无差异外 (P>0 .0 5) ,其余各组与 P2 组和对照组均有显著性差异 (P<0 .0 5) ;TNF- α含量在 P3 组最高 (P<0 .0 1 ) ;AE各组病人的 IFN- γ含量明显高于对照组 (P<0 .0 1 ) ,而 AE病人组间却并无差异 (P>0 .0 5)。低水平的 s IL-

In order to approach relationship among alveolar echinococcosis (AE) pathology and level of sIL 2R,TNF α and IFN γ in sera and significance of cytokines in development of AE, after 25 patients with AE were confirmed by ELISA and ultrasound, their sera were collected and the concentrations of sIL 2R,TNF α and IFN γ were detected by double antibody sandwich. Twelve individual health adults were served as controls, according to the lesion status of livers of AE patients recorded by ultrasound scanning, then they were divided into 4 groups, that is, P 2, P 3, P 4 and C (control group) . Average of concentrations of sIL 2R, TNF α and IFN γ of homologous group were statistically analyzed by both ANOV and Newman keuls respectively. The mean of sIL 2R of P 2 group was 96.57 u/mL; P 3: 226.44 u/mL; P 4: 193.81u/mL; control group: 110.89u/mL; the mean of TNF α of P 2 group was 1.12 μg/L; P 3:3.67 μg/L; P 4: 1.30 μg/L; control group 0.40 μg/L; the mean of IFN γ of P 2 group was 360 ng/L; P 3: 486 ng/L; P 4:259 ng/L; control group 15.63 ng/L; judgement with ANOV and newman keuls, the mean of concentrations of sIL 2R, TNF α and IFN γ had significant difference in different groups(P<0.01). Except for P 2 group, the mean of sIL 2R of other groups of AE patients had significant difference (P<0.05); the mean of TNF α concentration of P 3 group was the highest in all groups (P<0.01); the mean of IFN γ concentration of all patients was higher than that of control group(P<0.01), but had no difference between AE groups (P> 0.05). It could be concluded that low level sIL 2R indicate early stage of AE or in stable status, per contra, in evolution stage; TNF α of higher level be mixed in lesion of liver; role of single IFN γ be limited for immunological defense against AE and it can′t block pathological evolution.