乳香诱导急性非淋巴细胞白血病细胞凋亡中对Bcl-2基因调节

Boswellia Carterii Birdw Extractive Induces Apoptosis of Acute Non-lymphocytic Cells with Regulation of Expressions of Bcl-2 gene Protein

研究乳香诱导急性非淋巴细胞白血病细胞凋亡中对Bcl 2基因蛋白的影响。采用DNA片段百分率及免疫组化法检测 30例急性非淋巴细胞白血病细胞及白血病细胞株HL6 0细胞经 10 0 μg/ml乳香处理前、后Bcl 2基因蛋白表达水平。结果 :乳香具有诱导急性非淋巴细胞白血病细胞及HL6 0细胞凋亡[1] 及下调Bcl 2基因蛋白表达水平。结论 :乳香具有诱导急性非淋巴细胞白血病细胞及HL6 0细胞凋亡作用及下调Bcl 2基因蛋白。Bcl 2基因蛋白表达水平下调可能是乳香提取物诱导急性非淋巴细胞白血病细胞凋亡重要机制之一。

This study is to investigate the effects of Boswellia Carterii Birdw extractive (BCBE) on apoptosis of acute non lymphocytic leukemic (ANLL) cells and on the expression of Bcl 2 gene protein.Methods:The target leukemia cell line HL60 and bone marrow leukemic cells from 30 ANLL patients were studied under the treatment of 100 μg/ml BCBE.Apoptosis was detected by percentage of DNA fragmentation and Bcl 2 gene protein expression by immunohistochemistry test.Results:BCBE induced apoptosis of ANLL cells and HL60 cells,and down regulation of the expression of Bcl 2 gene protein.Conclusion:Down regulation of the expression of Bcl 2 gene protein level may be part of the mechanism of BCBE inducing the apoptosis of acute non lymphocytic leukemic cells.